文章摘要
张定国,张馥敏,张郁青,高 翔.促红细胞生成素对大鼠骨髓间充质干细胞增殖和细胞周期的影响[J].南京医科大学学报,2007,(2):142~144162
促红细胞生成素对大鼠骨髓间充质干细胞增殖和细胞周期的影响
Effects of erythropoietin on proliferation and cell circle stage of rat bone marrow mesen-chymal stem cells in vitro
投稿时间:2006-06-15  
DOI:10.7655
中文关键词: 促红细胞生成素  骨髓间充质干细胞  增殖  细胞周期
英文关键词: erythropoietin  mesenchymal stem cells  proliferation  cell circle stage
基金项目:
作者单位
张定国 南京医科大学第一附属医院心脏科江苏 南京 210029 
张馥敏 南京医科大学第一附属医院心脏科江苏 南京 210029 
张郁青 南京医科大学第一附属医院心脏科江苏 南京 210029 
高 翔 南京大学模式动物研究所江苏 南京 210008 
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中文摘要:
       目的: 观察促红细胞生成素(eryrthropoietin, EP)对大鼠骨髓间充质干细胞(mesenchymal stem cells, MSCs)增殖和细胞周期的影响。方法:无血清条件下,不同剂量(0, 0.25, 0.5, 1, 2, 10 U/ml)EP与MSCs共培养24 h,MTT方法检测MSC增殖情况;10 U/ml EP与MSCs共培养72 h后,流式细胞仪检测细胞周期情况。结果: MTT结果表明,EP呈剂量依赖性促进MSCs增殖,除0.25 U/ml剂量外,其余各组都与对照组有显著性差异,以10 U/ml EP促MSCs增殖作用最明显。流式细胞检测表明, EP组能降低G0/G1期比例,增加S 期和G2/M 期细胞比例,与对照组相比,差异均有显著性意义(P < 0.05)。结论: EP可以促进骨髓间充质干细胞增殖,并且这种影响呈剂量依赖性。
英文摘要:
      Objective:To determine the effect of erythropoietin(EP) on the proliferation of mesenchymal stem cells(MSCs) in vitro. Methods:Under serum free culture media, MSCs were incubated with different doses of EP for 24 h(0, 0.25, 0.5, 1, 2, 10 U/ml), and then the proliferation of MSCs was measured by MTT assay. Cell circle stage was measured by flow cytometry assays after incubation of 10 U/ml EP for 72 h. Results:MTT assay showed that EP could enhance MSCs proliferation in a dose-dependent manner. There was significant difference among EP treatment groups and control group,except 0.25 U/ml group. The effects on the proliferation of MSCs was highest in 10 U/ml group. Furthermore,flow cytometry(FCM) assays showed that, compared with control group, EP could significantly decrease G0/G1 ratio, and increase S stage and G2/M stage ratio. Conclusion:EP could enhance MSCs proliferation in dose-dependent manner.
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