文章摘要
陆晓梅,王 淼,赵人琤.高效液相法测定兔眼房水中醋甲唑胺的含量[J].南京医科大学学报,2007,(5):457~459
高效液相法测定兔眼房水中醋甲唑胺的含量
Determination of methazolamide in aqueous humor of rabbit eyes by HPLC
投稿时间:2006-12-19  
DOI:10.7655
中文关键词: 高效液相色谱法  眼房水  醋甲唑胺
英文关键词: HPLC  aqueous humor  methazolamide
基金项目:
作者单位
陆晓梅 南京医科大学公共卫生学院卫生检验系江苏 南京 210029 
王 淼  
赵人琤  
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中文摘要:
      目的:用高效液相(HPLC)法测定兔眼房水中醋甲唑胺浓度,提供其药动学依据。方法:以10%三氯醋酸沉淀兔眼房水中的蛋白质,采用μ1BondapakTM C18柱(3.9 mm × 300 mm,10 ?滋m),流动相为甲醇 ∶ 水(25 ∶ 75,V/V),检测波长为290 nm,流速1 ml/min,柱温40℃。结果:兔眼房水中醋甲唑胺浓度在0.05~10.00 ?滋g/ml范围内呈良好的线性关系,r = 0.9982,方法的相对回收率101.7%,绝对回收率82.7%,日内和日间RSD小于10%。结论:高效液相法灵敏度高,操作简单快速,结果可靠,适合于眼房水醋甲唑胺药动学研究。
英文摘要:
      Objective:To establish a practical HPLC method to determine the concentration of methazolamide in aqueous humor of rabbit eyes for the research of methazolamide drug delivery system. Method:Protein was deposited by 10%(V/V) trichloroacetic acid. Methazolamide was extracted from aqueous humor by using C18 column and mobile phase of methanol ∶ water(25 ∶ 75,V/V). The column temperature was maintained at 40℃, methazolamide was detected at 290 nm, and the flow rate was 1.0 ml/min. Results:A good linear relationship was obtained between the concentration of methazolamide from 0.05 ?滋g/ml to 10 ?滋g/ml, r = 0.9982. The Relative recovery and absolute recovery of methazolamide were 101.7% and 82.7%, respectively; and RSD of intra-day and inter-day were less than 10%. Conclusion:The improved method could significantly enhance the detective sensitivity without damage to the column and increase the accuracy. The procedure was simple, sensitive, and suitable for determination of methazolamide in aqueous humor with good practicability.
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