文章摘要
闵笑颜,钱 波,刘 莉,张小进,程蕴琳.内毒素诱导小鼠心肌中Toll样受体4通路分子靶点激活的时间相关性[J].南京医科大学学报,2007,(6):538~541
内毒素诱导小鼠心肌中Toll样受体4通路分子靶点激活的时间相关性
The time correlation with the activation of molecule targets in Toll like receptor 4 signaling parthway in mice myocardium activated by LPS
投稿时间:2006-11-08  
DOI:10.7655
中文关键词: 心肌  TLR4通路  内毒素  小鼠
英文关键词: mice myocardium  TLR4 signaling  lipopolysaccharide  mice
基金项目:江苏省卫生厅“135工程”重点科学基金资助项目(135214);南京医科大学创新基金(CX2003018)
作者单位
闵笑颜 南京医科大学第一附属医院老年医学科江苏 南京〓210029 
钱 波  
刘 莉  
张小进  
程蕴琳  
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中文摘要:
      目的:探讨内毒素(lipopolysaccharide, LPS)诱导的小鼠心肌中Toll样受体4 (Toll like receptor 4,TLR4)通路分子靶点激活的时间相关性及其意义。方法: 将雄性C57BL/6J小鼠随机分为LPS处理组(腹腔注射LPS 10 mg/kg)和生理盐水对照组,Western blotting检测处理后5 min、10 min、30 min、1 h、2 h、6 h、12 h、24 h各时间点(n = 3) 心肌TLR4通路各分子靶点的激活。结果:TLR4-MyD88-IκBα通路5 min即被激活,2 h达高峰,随后开始下降(P < 0.05)。MAPKs 5 min被激活,6 h达峰值,随后开始下降(P < 0.05)。Akt 10 min被激活,6 h达峰值,其后逐渐下降(P < 0.05)。结论:LPS诱导心肌TLR4通路的激活与时间变化存在密切联系。 
英文摘要:
       Objective:To investigate the time correlation with the activation of molecule targets in Toll Like Receptor 4(TLR4) signaling in mice myocardium activated by lipopolysaccharide(LPS) and its significance. Methods:Male C57BL/6J mice were randomly divided into LPS group(intraperitoneal injection with LPS 10 mg/kg) and saline control group. The expression of molecule targets in TLR4 signaling parthway were detected at 5 min,10 min,30 min,1 h,2 h,6 h,12 h,24 h(n = 3) after treatment by Western blotting. Results:The expression of TLR4-MyD88-IκBα signaling is activated 5 min after injection, reached the peak at 2 h, then decreased(P < 0.05). The expression of MAPKs is activated 5 min after injection, reached the peak at 6 h, then decreased(P < 0.05). The expression of Akt is activated 10 min after injection, reached the peak at 6 h, then decreased(P < 0.05). Conclusion: There is a close correlation between the activation of TLR4 signaling and the time of LPS treatment in mice myocardium .
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