文章摘要
张长和,李君,葛文刚,刘现忠,仲跻巍,李相成.普乐可复对肝癌细胞株HepG2增殖的影响[J].南京医科大学学报,2007,(6):565~568
普乐可复对肝癌细胞株HepG2增殖的影响
The effect of prograf on the hepatocellular carcinoma cell line HepG2
投稿时间:2006-11-25  
DOI:10.7655
中文关键词: 普乐可复  肝癌细胞  增殖  TGF-α  C-met
英文关键词: FK506  hepatocellular carcinoma cell  proliferation  TGF-α  C-met
基金项目:
作者单位
张长和 南京医科大学第一附属医院肝脏外科江苏 南京 210029 
李君  
葛文刚  
刘现忠  
仲跻巍  
李相成  
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中文摘要:
      目的:研究普乐可复(即FK506)对肝癌肝移植术后体内可能残存肿瘤细胞的影响,从而对肝癌患者肝移植术后抗排异药物的选择起一定的指导作用。方法:在体外细胞培养条件下,通过MTT、RT-PCR、Western blot方法,研究普乐可复对肝癌细胞株HepG2增殖及其肿瘤相关细胞因子TGF-α和C-met的特异性表达的影响。结果:普乐可复组(5 μg/L) 对肝癌细胞株HepG2增殖及TGF-α及c-Met特异mRNA及蛋白的表达有抑制作用。与对照组相比,差异均有统计学意义(P < 0.05)。结论:FK-506对肝癌细胞生长有一定的抑制作用。
英文摘要:
      Objective:To investigate the effect of prograf on remnant hepatocellular carcinoma cells in the liver receptors after liver transplantation for liver cancer, and to instruct the anti-rejection drug selection. Methods:MTT assay,RT-PCR and Western blot were used to examine the expression of c-met and TGF-α in the hepatocellular carcinoma cell line HepG2 cultured in vitro. Results:The expression of c-met and TGF-αin prograf group(at concentration of 5 μg/L) was much lower than that in the control group(P < 0.05). Conclusion:Prograf can inhibit the proliferation of hepatocellular carcinoma cell.
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