Kai-1基因外显子缺失与大肠癌的临床关系
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江苏省卫生厅自然科学基金重点资助项目(H2009)


Relationship between loss of exon 9 of Kai-1 gene and clinicopathological significance in colorectal cancer
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    摘要:

    目的:探讨Kai-1基因的缺失在结直肠癌演进与转移中的意义。方法:提取40例结直肠癌患者的肿瘤组织(24例无淋巴转移,16例有淋巴转移),15例癌旁正常组织的RNA,RT-PCR扩增,电泳检测,测序验证Kai-1基因的缺失情况。结果:40例组织标本中18例出现Kai-1基因exon 9缺失(16例杂合缺失,2例纯合缺失),15例癌旁正常组织中有2例出现Kai-1基因exon 9缺失(2例杂合缺失);在大肠癌组织中Kai-1基因缺失频率显著高于在癌旁正常组织中(P < 0.05);在有淋巴转移的结直肠癌组织中,缺失的频率明显高于无淋巴转移的结直肠癌组织(P < 0.05),在大肠癌中晚期(DukesC期)明显高于早期(DukesA-B期)(P < 0.05),而Kai-1基因的缺失频率与结直肠癌患者的年龄、性别、组织学类型以及分化程度无关 (P > 0.05)。结论:Kai-1基因缺失可能与在大肠癌演进、转移有关,检测其缺失可作为判断大肠癌的演进与转移的客观临床指标。

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    Objective:To explore the significance loss of Kai-1 gene plays in the progression and metastasis of colorectal cancer (CRC). Methods:The deletion of Kai-1 gene were detected by using RT-PCR and validated by sequencing in the tumor of 40 cases of CRC and 15 normal cases near the tissues of CRC. Results:The deletion of exon 9 in the Kai-1 gene was found in 18 of 40 colorectal cases(45%) and in 2 of 15 normal cases near the colorectal tissues(13.3%). The frequency of the exon 9 deletion was significantly higher in the colorectal tumors than in the normal cases(P < 0.05). The frequency of the exon 9 deletion was significantly higher in the colorectal tumors with lymphatic metastasis than that without lymphatic metastasis(P < 0.05), and in the advanced stage(Dukes C,D) than in the earlier stage(Dukes A,B) of the colorectal cancer(P < 0.05). However, there was no significant difference of the frequency of exon 9 deletion when stratefied by the the age, sex, histological type as well as differentiated degree of the patients with CRC(P > 0.05). Conclusion:The deletion of the Kai-1 was associated with the progression and metastasis of CRC, and the detection of the deletion of the Kai-1 can be considered as an objective clinical marker to assess the progression and metastasis of CRC.

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陈刚,陈森清,张晓梅,尚俊清,周建农.Kai-1基因外显子缺失与大肠癌的临床关系[J].南京医科大学学报(自然科学版),2007,(6):594-597

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  • 收稿日期:2006-11-24
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