文章摘要
王建立,唐金荣,苏建华,侯熙德,张平,朱学江,吴乐,肖杭.改善神经微循环对受压大鼠坐骨神经VEGF表达及神经传导速度的影响[J].南京医科大学学报,2007,(6):598~601
改善神经微循环对受压大鼠坐骨神经VEGF表达及神经传导速度的影响
The influences on expression of VEGF and nervous conduction by improving microcircu-lation following crushing lesion of sciatic nerve in rats
投稿时间:2006-12-07  
DOI:10.7655
中文关键词: 坐骨神经嵌压性损害  微循环  足趾间距  神经传导  内皮细胞生长因子
英文关键词: sciatic nerve crush injury  microcirculation  distance between toes  nervous conduction  vascular endothelium growth factor
基金项目:江苏省自然科学基金资助项目(BK2001116),常州市卫生局资助项目(200417)
作者单位
王建立 南京医科大学第一附属医院神经内科江苏 南京 210029 
唐金荣 南京医科大学第一附属医院神经内科江苏 南京 210029 
苏建华 南京医科大学第一附属医院神经内科江苏 南京 210029 
侯熙德 南京医科大学第一附属医院神经内科江苏 南京 210029 
张平 南京医科大学第二附属医院病理科江苏 南京 210011 
朱学江 南京医科大学基础医学院生理教研室江苏 南京 210029 
吴乐 南京医科大学基础医学院神经毒理研究所江苏 南京 210029 
肖杭 南京医科大学基础医学院神经毒理研究所江苏 南京 210029 
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中文摘要:
      目的:探讨改善微循环对周围神经嵌压性损害血管内皮细胞生长因子(VEGF)表达和神经传导功能的影响。方法:制作坐骨神经嵌压性损害的动物模型,在不同时点采用免疫组化与行为医学、电生理学的方法检测内皮细胞生长因子水平与大鼠足趾间距、坐骨神经传导速度,并将各项检测结果进行对比分析。结果:① 嵌压大鼠坐骨神经后,12 h起各组背根神经节细胞VEGF表达开始增加,72 h达到高峰,与正常对照组比较,其余各组各时段背根神经节细胞VEGF表达水平均显著增加(P < 0.01/P < 0.05),前列地尔组和盐酸丁咯地尔组较模型组为优(P < 0.01/P < 0.05)、表达的数量增加、持续时间延长。②4周时,前列地尔组和盐酸丁咯地尔组第4周时大鼠足趾间距、坐骨神经传导速度虽较正常对照组差(P < 0.01/P < 0.05),但均较模型组为优(P < 0.05)。结论:周围神经嵌压性损害后神经受损,改善微循环可增加VEGF的表达并提高神经传导速度、减轻神经功能损害,因而对周围神经嵌压性损害的修复具有促进作用。
英文摘要:
      Objective:To study the influences on expression of vascular endothelium growth factor(VEGF) and nervous conduction by improving microcirculation following crushing lesion of sciatic nerve in rats. Methods:Sciatic nerve was crushed used pincers to establish the model of sciatic nerve crush lesion in rats. The expression of VEGF in dorsal ganglion cells at 12 h,72 h,7 th days and the distance between right behind toes, nerve conduction velocity of sciatic nerve at 4th week were examined respectively by immunohistochemical and electrophysiologic techniques. Then their results were compared with each other. SD rats were divided into control group, model group, alprostadil(Lipo-PGE1) group and buflomedil hydrochloride group at random. There were 16 rats in each group. SPSS 10.0 was used statistically. Results:①The expression of VEGF in dorsal ganglion cells increased from 12 h after crush injury of the nerve, and have increased to the highest at 72 h after crush injury of the nerve(P < 0.01). There was no increasing of it in normal group. It increased in alprostadil group and buflomedil hydrochloride group more than model group significantly(P < 0.01). It became normal on 1 th week in all groups after sciatic nerve crush injury. ②On 4th week after sciatic nerve crush injury, distance between right behind toes and nervous conduction velocity of sciatic nerve in alprostadil group and buflomedil hydrochloride group were more optimal than those in model group(P < 0.01) and no more optimal than those in normal group(P < 0.05), while there was normal in control group. Conclusion:The expression of VEGF increase and the nervous conduction velocity of sciatic nerve elevate by improving microcirculation significantly after sciatic nerve crush lesion, so that it has improving action to repair peripheral nerve crush lesion.
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