文章摘要
邹 蓉,陈 平,金文杰,黄亚辉,钱燕宁.体外比较硫贲妥钠、咪唑安定对成年人Th0细胞分化的影响[J].南京医科大学学报,2007,(6):602~605
体外比较硫贲妥钠、咪唑安定对成年人Th0细胞分化的影响
Effects of pretreatment with thiopental and midazolam on the differentiation of human helper T cells in vitro
投稿时间:2006-11-20  
DOI:10.7655
中文关键词: 硫贲妥钠  咪唑安定  Th1/Th2  细胞因子  流式细胞技术
英文关键词: thiopental  midazolam  Th1/Th2  cytokine  flow cytometry
基金项目: 南京医科大学创新基金资助项目(MC0005),江苏省医学重点人才基金资助项目(RC2002058)
作者单位
邹 蓉 南京医科大学第一附属医院麻醉科江苏 南京 210029 
陈 平  
金文杰  
黄亚辉  
钱燕宁  
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中文摘要:
      目的:体外比较不同浓度硫贲妥钠、咪唑安定对成年人Th0细胞分化的影响。方法:采集20例健康志愿者外周静脉血,进行全血和外周血单个核细胞(PBMCs)培养。每一份血样均用于下列各组研究:①空白对照组(C组);②不同浓度硫贲妥钠组[4 μg/ml(T1组)、20 μg/ml(T2组)和40 μg/ml(T3组)];③不同浓度咪唑安定组[50 ng/ml(M1组)、150 ng/ml(M2组)和500 ng/ml(M3组)]。培养的全血和PBMCs均用相应浓度药物预先作用24 h,然后加入相应的刺激剂。刺激后检测胞内细胞因子(IFN-γ+/IL-4+)和细胞膜表面趋化因子受体(CCR5+/CCR3+)含量评估Th0细胞的分化情况。结果:分别与C组和T1组相比较,T2、T3组的Th1细胞亚群比例均明显降低(P < 0.05);与T1组相比较,T3组的Th2细胞亚群比例明显降低(P < 0.05)。咪唑安定各组Th1、Th2细胞亚群的比例无显著改变。结论:硫贲妥钠对Th1细胞分化有明显抑制作用,使Th0细胞向Th2方向分化;咪唑安定对Th0细胞分化无明显影响。
英文摘要:
      Objective:To observe the effect of different concentrations of thiopental and Midazolam on the differentiation of T helper cells in vitro in adults. Methods:Peripheral venous blood samples were collected from 20 middle-aged healthy donors. In each volunteer,20 ml of blood was divided into 7 groups:control group,3 thiopental groups(4 μg/ml,20 μg/ml, 40 μg/ml),3 midazolam groups(50 ng/ml, 150 ng/ml, 500 ng/ml). Whole blood and mononuclear cells from peripheral blood(PBMCs) were incubated with different concentrations of thiopental and midazolam for 24 h, while the control group without any drugs. Then the stimulants- phorbolmyristate+lonomycin+glogistop were added to whole blood and phytohemagglutinin(PHA) was add to PBMCs. The whole blood was incubated for another 4 h and PBMCs were incubated for another 48 h. Then the T-lymphocytes were collected for determination of intracellular level of IFN-γ(as a marker of Th1 cells) and IL-4(as a marker of Th2 cells) in the whole blood using flow cytometry and the expression of CCR5+(as a marker of Th1 cells) and CCR3+(as a marker of Th2 cells) in PBMCs. Results:Compared with the control group, in thiopental 20 μg/ml, 40 μg/ml group, the percentage of Th1 cells decreased significantly. Among different concentration in thiopental groups, the percentage of Th1 cells in high-dose or middle-dose group was obviously lower than that in low-dose group, the percentage of Th2 cells in high-dose was obviously lower than that in low-dose group. In midazolam group, there was no significant change. Conclusion:Thiopental can inhibit Th1 activity and shift the balance towards immunosuppressive Th2-like responses predominantly. Midazolam has no significant effect on Th1 and Th2 activity.
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