文章摘要
薛 莹,刘翠萍,袁庆新,徐宽枫,刘 超.大鼠妊娠期胰岛形态及β细胞增殖能力的变化[J].南京医科大学学报,2007,(7):698~701
大鼠妊娠期胰岛形态及β细胞增殖能力的变化
Adaptation on the morphology and proliferation of pancreatic islet during pregnancy in rats
投稿时间:2007-01-01  
DOI:10.7655
中文关键词: 妊娠  胰岛  增殖
英文关键词: pregnancy  pancreatic islet  proliferation
基金项目:江苏省医学重点学科资助项目(SK200214)
作者单位
薛 莹 南京医科大学第一附属医院内分泌科,江苏 南京 210029 
刘翠萍  
袁庆新  
徐宽枫  
刘 超  
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中文摘要:
      目的:探讨大鼠妊娠不同阶段胰岛形态及β细胞增殖的变化?方法:采用5-溴尿脱氧嘧啶核苷(BrdU)及抗胰岛素抗体免疫组化双染法,通过形态学及量化分析,研究大鼠妊娠10.5?12.5?14.5?18.5和20.5天时胰岛形态的改变及β细胞的增殖率的改变?结果:与正常对照组相比, 大鼠妊娠12.5天时胰岛素染色阳性区域平均光密度?胰岛素相对浓度(IRC)?胰岛素阳性表达细胞密度(PCD)以及胰岛β细胞增殖指数(PI)均明显增高,并于孕14.5天到达高峰,而后逐渐下降至对照组水平?此外,大鼠妊娠14.5天至20.5天时胰岛素阳性表达细胞面积亦明显高于正常水平?结论:大鼠妊娠期胰岛β细胞的增殖能力和胰岛素表达,均从孕12.5天开始发生改变,孕14.5天变化最为显著,并持续至孕晚期(孕20.5天)?
英文摘要:
      Objective:To elucidate the adaptive changes on the morphology and proliferation of pancreatic islet during pregnancy in rats. Methods:Rat pancreatic tissues of each group were double-stained by 5-bromodeoxyuridine(BrdU) and anti-insulin antibody by immunohistochemistry technique to measure the changes of islet morphology and quantity during pregnancy at day 10.5,12.5, 14.5,18.5 and 20.5. Results:Compared with the normal control group, the average photodensity of insulin-expression positive zone, insulin relative concentration(IRC), insulin-expression positive cell density(PCD) and proliferation index(PI)of pancreatic islet were all significantly increased on day 12.5, rose continuously to a peak at day 14.5 of pregnancy, and then gradually returned to levels as control. In addition, areas of insulin-expression positive cells were also much greater during pregnancy during day 14.5 to day 20.5 than in non-pregnant rats. Conclusion:Both pancreatic islet ?茁 cell proliferation and insulin-expression concentration became more significantly increased than sex-matched virgin control rats from day 12.5, peaked at day 14.5 of pregnancy,and lasted to late pregnancy(day 20.5).
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