文章摘要
陈 凝,杨 觅,钱晓萍,禹立霞,刘宝瑞.紫杉醇脂质体腹腔给药对S180腹水瘤小鼠的疗效[J].南京医科大学学报,2007,(7):706~710
紫杉醇脂质体腹腔给药对S180腹水瘤小鼠的疗效
Antitumor effects of paclitaxel-loaded liposome on S180 ascites-tumour bearing mice
投稿时间:2007-02-25  
DOI:10.7655
中文关键词: 紫杉醇  脂质体  腹水  腹腔转移
英文关键词: paclitaxel  liposome  ascites  metastasis in abdominal cavity
基金项目:江苏省卫生厅项目(H200349)
作者单位
陈 凝 南京大学医学院附属鼓楼医院 肿瘤中心,江苏 南京 210008 
杨 觅  
钱晓萍  
禹立霞  
刘宝瑞  
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中文摘要:
      目的:比较紫杉醇脂质体与紫杉醇注射液对S180腹水瘤小鼠的肿瘤抑制效果?方法:采用逆向蒸发法及超声法制备紫杉醇脂质体,以激光粒度散射仪测定粒径,以反向高效液相法测定脂质体中紫杉醇药物含量?采用S180细胞昆明鼠腹腔内注射形成腹水瘤模型,肿瘤种植24 h后随机分成4组,分别为空白对照组?空白脂质体组?紫杉醇注射液组?紫杉醇脂质体组?每组5只,其中两组分别以紫杉醇脂质体和紫杉醇注射液腹腔注射,其他2组分别给予生理盐水和空白脂质体腹腔注射,共给药4次,给药间隔72 h?结果:紫杉醇脂质体平均粒径为(282.40 ± 8.94)nm,药物包封率为91%?肿瘤种植14天后,空白对照组?空白脂质体组?紫杉醇注射液组小鼠形成大量腹水,腹膜?肝脏广泛转移;紫杉醇脂质体组仅形成少量腹水,腹膜?肝脏未见转移?结论:紫杉醇脂质体腹腔内给药可以明显抑制腹水形成,有效抑制腹腔转移?
英文摘要:
      Objective:To compare the antitumor effect of paclitaxel-loaded liposomes with paclitaxel on S180 ascites-tumour bearing mice. Methods:The paclitaxel-loaded liposomes were prepared by reverse evaporation and sonication techniques. The particle diameters were measured by dynamic light scattering(DLS) measurement and the entrapment efficiency was measured by the reverse-phase high-pressure liquid chromatograph(RP-HPLC) method. Twenty Kunming mice bearing ascites tumor xenografts were distributed into four groups at random:the control,empty-liposomes,free paclitaxel group and the paclitaxel-loaded liposomes. Treatments were started after 24 hours of implantation. Two groups bearing S180 xenograft were treated i.p. with 10 mg/kg of paclitaxel(free drug or liposome encapsulated). The other two groups were given i.p. with saline or empty-liposomes. All the treatments were given 4 times and the interval was 72 hours. Results:The average diameter of the paclitaxel-loaded liposome was 282.4 ± 8.94 nm. The entrapment efficiency was 91%. There were resembly much ascites in the three groups of the control, empty-liposomes and free paclitaxel. While in the group of paclitaxel-loaded liposomes,there was little ascites. In addition,there were dramatically little metastasis in abdominal cavity with the group of the paclitaxel-loaded liposomes other than the remain three groups. Conclusion:Ascites and metastasis in abdominal cavity could be inhibited by paclitaxel-loaded liposomes to S180 ascites-tumour bearing mice remarkly.
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