Objective:To detect the expression of placenta growth factor(PlGF) in maternal serum and urine from preeclampsia (PE), and to identify whether the level of PlGF in urine is significantly correlative with that in serum. Methods:Women enrolled prospectively in following groups: healthy pregnant control(n = 20) and PE patients, which contained mild preeclampsia(n = 20)-severe preeclampsia(n = 15). The serum and urinary PlGF levels were measured by enzyme linked immunoassay(ELISA). Results: The serum PlGF levels in severe preeclampsia group(23.40 ± 9.88)pg/ml were significantly lower than that of normal pregnancies(44.04 ± 19.26)pg/ml and mild preeclampsia group(36.13 ± 17.29)pg/ml. The urinary PlGF level in severe preeclampsia group(587.20 ± 381.86)pg/mmol was also significantly lower than that in normal pregnancies(961.65 ± 584.58)pg/mmol and mild pre-eclampsia group(939.20 ± 474.94)pg/mmol. The urinary PlGF level was significantly correlative with that in serum(r = 0.713). Conclusion:Severe preeclampsia is associated with the low serum and urinary placenta growth factor levels in late pregnancy. Urinary PlGF level is significantly correlative with serum PlGF level. The level of PlGF in urine may be used to study the connection between PE and PlGF instead of serum PlGF level and offer a more convenient method to make a diagnosis, which may guide the clinical treatment of PE.