文章摘要
张国英,王淑玉.一种L-选择素配体合成酶在子宫内膜癌和正常子宫内膜中的差异表达[J].南京医科大学学报,2007,(7):718~720732
一种L-选择素配体合成酶在子宫内膜癌和正常子宫内膜中的差异表达
Different expression of a synthetic enzymen for L-selectin ligant in the endometrium from the patients with carcinoma of endometrium and normal women
投稿时间:2007-01-03  
DOI:10.7655
中文关键词: 子宫内膜  子宫内膜癌  N-乙酰氨基葡萄糖-6-磺基转移酶
英文关键词: endometrium  carcinoma of endometrium  N-acetyl-glucosamine-6-O-sulfotransferase
基金项目:
作者单位
张国英 南京医科大学第一附属医院妇产科,江苏 南京 210029 
王淑玉  
摘要点击次数: 1368
全文下载次数: 99
中文摘要:
      目的:检测L-选择素配体合成的关键酶,N-乙酰氨基葡萄糖-6-磺基转移酶(N-acetyl-glucosamine-6-O-sulfotransferase),在子宫内膜癌和正常子宫内膜组织中的表达?方法:采用RT-PCR技术检测16例子宫内膜癌(研究组)和16例正常子宫内膜(对照组)组织中N-乙酰氨基葡萄糖-6-磺基转移酶的表达?结果:正常子宫内膜有N-乙酰氨基葡萄糖-6-磺基转移酶的表达; 其表达的半定量值,癌组织为0.87 ± 0.08,正常组织为0.45 ± 0.11,癌旁组织为0.49 ± 0.12,癌组织中明显高于正常子宫内膜,两组差异有显著性(P < 0.001);癌组织中明显高于癌旁组织,两组差异有显著性(P < 0.001)?结论:L-选择素配体合成酶—N-乙酰氨基葡萄糖-6-磺基转移酶,在子宫内膜癌中的高表达,可能与肿瘤的发生?发展和转移密切相关?
英文摘要:
      Objective:To investigate the expression of N-acetyl-glucosamine-6-O-sulfotransferase,an important enzyme in synthetic enzyme for L-selectin ligant in the endometrium from the patients with carcinoma of endometrium and normal women. Methods:Reverse transcription polymerase chain reaction(RT-PCR) method was used to detect and quantify N-acetyl-glucosamine-6-O-sulfotransferase mRNA expression levels in the endometrium of 16 patients with carcinoma of endometrium which were recruited as study group and 16 healthy women as the control. Results:The expression of N-acetyl-glucosamine-6-O-sulfotransferase was observed in normal, but the expression level in study group was significantly higher than that in control group(0.87 ± 0.08 vs 0.45 ± 0.11,P < 0.001), higher than that in paraneoplastic endometrium of the patients with carcinoma(0.87 ± 0.08 vs 0.49 ± 0.12,P < 0.001). Conclusion:The higher expression of N-acetyl-glucosamine-6-O-sulfotransferase in the endometrium from the patients with carcinoma of endometrium may indicate a close correlation with the development and metastasis of cancer cells.
查看全文   查看/发表评论  下载PDF阅读器