文章摘要
单晓红,许 璇,胡玉龙,王永梅,范乐明,陈 琪,李跃华.缺血再灌注对心肌细胞转录因子GATA?鄄4表达的影响[J].南京医科大学学报,2007,(8):785~788
缺血再灌注对心肌细胞转录因子GATA?鄄4表达的影响
The effect of myocardial ischemia-reperfusion on the expression of transcription factor GATA-4 in cardiac muscle cells
投稿时间:2007-01-29  
DOI:10.7655
中文关键词: 心肌缺血  缺血再灌注损伤  核转录因子GATA-4
英文关键词: myocardial ischemia  ischemia-reperfusion injury  transcriptional factor GATA-4
基金项目:江苏省自然科学基金资助项目(BK2006229)
作者单位
单晓红 南京医科大学病理生理学系,江苏 南京 210029 
许 璇  
胡玉龙  
王永梅  
范乐明  
陈 琪  
李跃华  
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中文摘要:
      目的:观察缺血再灌注(ischemia/reperfusion, I/R)对心肌核转录因子GATA?鄄4影响?方法:健康清洁级Balb/c小鼠16只,分为假手术组及实验组(每组8只)?通过结扎冠状动脉左前降支建立心肌缺血模型, 45 min后恢复心肌血液灌注?4 h后M型超声心动图测定心脏功能?取心脏标本测定GATA?鄄4基因和蛋白表达,同时做组织病理切片观察心肌组织的形态结构改变?结果:I/R导致心脏功能受损?光镜下心肌组织结构异常,炎症细胞浸润明显?与假手术组比较,实验组GATA?鄄4基因和蛋白表达明显降低(P < 0.05)?结论:缺血再灌注引起心脏功能下降,同时下调GATA?鄄4表达,提示GATA?鄄4蛋白可能与心肌缺血再灌注损伤的发生有关?
英文摘要:
      Objective:To investigate the effect of myocardial ischemia-reperfusion on the expression of transcription factor GATA-4. Methods:Sixteen adult healthy Balb/c mice were randomly divided into control group and experimental group. Heart ischemia was achieved by ligation of left anterior coronary descending branch. After 45 min ischemia, the ligation was removed for 4 h reperfusion. Echocardiography was used to determine the heart function. Heart samples were taken for further analysis. Results:I/R resulted in poor heart function, accompanying the downregulation of GATA-4 mRNA and protein expression. Histological examination showed there were more inflammatory cells in I/R heart. Conclusion:GATA-4 was modulated by I/R and probably responsible for I/R induced heart injury partially.
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