文章摘要
周 栋,邹良建,杨 勇.CBP基因在非小细胞肺癌中的表达及其生物学意义[J].南京医科大学学报,2007,(8):797~800863
CBP基因在非小细胞肺癌中的表达及其生物学意义
Reduction of CBP gene expression in NSCLC:expression and correlation with clinicopat-hologic parameters
投稿时间:2007-01-21  
DOI:10.7655
中文关键词: 非小细胞肺癌  CBP基因  Src羧基端激酶
英文关键词: non-small cell lung cancer  CSK-binding protein  C-terminal Src kinase
基金项目:国家自然科学基金资助项目(30270335)
作者单位
周 栋 第二军医大学长海医院胸心外科,上海 200433 
邹良建  
杨 勇  
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中文摘要:
      目的:研究CBP(CSK-binding protein)基因表达下降与人肺癌临床病理生理特征的关系,探讨CBP基因表达与肺癌肿瘤细胞发生?侵袭的相关性?方法:对50例非小细胞肺癌(NSCLC)标本(含癌旁组织)和10例肺良性病变组织应用单克隆抗体?免疫组化SP法检测CBP基因的蛋白表达情况?应用RT-PCR方法检测NSCLC中CBP的mRNA表达水平?结果:肺癌肿瘤组织中CBP基因表达水平显著低于癌旁组织和肺良性病变组织(P < 0.05)?肺癌组织中CBP基因表达水平降低与肿瘤分期?淋巴结转移程度存在相关性(P < 0.05),与肺癌组织学类型?细胞分化程度以及患者的性别?年龄无关?结论:CBP基因可能参与了对肺癌肿瘤细胞Src家族成员激酶活性的负反馈调节,其表达下降可能与肺癌的发生?发展过程有关?
英文摘要:
      Objective:The aim of this study was to assess the expression of protein products of CBP(CSK-binding protein) in non-small cell lung cancer(NSCLC), to estimate their possible expression and to correlate the results of immunohistochemical analysis with various clinicopathologic parameters. Methods:The expression of CBP gene was detected by immunohistochemical S-P method in 50 cases of NSCLC tissues,50 para-cancer tissues and 10 cases of lung benign lesion tissues as control. According to immunohistochemical results, the expressions of CBP mRNA were detected by reverse transcriptase polymerase chain reaction(RT-PCR) in corresponding samples. Results:The expression level of CBP gene was found to be significantly lower in NSCLC tissues than in para-cancer tissues and lung benign lesion tissues(P < 0.05). The expression level of CBP gene was closely related to PTNM stages and lymph node involvement in Lung cancer patients(P < 0.05). Conclusion:CBP gene may play a role in the negative feedback regulation of Src-family kinases in non-small cell lung cancer, and reduction of CBP gene expression may be related to the pathogenesis and development of NSCLC.
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