文章摘要
朱华云,刘凌翔,葛红梅,王同杉,刘 平.NF-κB抑制剂PDTC对人胃癌SGC-7901细胞凋亡及Livin和Caspase-3表达的影响[J].南京医科大学学报,2007,(8):816~820
NF-κB抑制剂PDTC对人胃癌SGC-7901细胞凋亡及Livin和Caspase-3表达的影响
The effect of PDTC on apoptosis and expression of Livin and Caspase-3 on human gastric carcinoma cell SGC-7901
投稿时间:2006-11-20  
DOI:10.7655
中文关键词: 吡咯烷二硫代氨基甲酸盐  胃癌  细胞凋亡  核因子-κB  Livin  Caspase-3
英文关键词: PDTC  human gastric carcinoma  apoptosis  NF-κB  Livin  Caspase-3
基金项目:江苏省医学重点人才“135”工程资助项目(52-2001)
作者单位
朱华云 南京医科大学第一附属医院肿瘤科,江苏 南京 210029 
刘凌翔  
葛红梅  
王同杉  
刘 平  
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中文摘要:
      目的:观察核因子-?资B(NF-κB)抑制剂吡咯烷二硫代氨基甲酸盐(PDTC)对人胃癌SGC-7901细胞生长及凋亡的影响,研究其对凋亡抑制蛋白Livin和Caspase-3表达水平的调控,探讨PDTC对人胃癌SGC-7901细胞凋亡影响的机制?方法:不同浓度的PDTC作用于SGC-7901细胞不同时间后,MTT法测定其对SGC-7901细胞增殖的抑制率;流式细胞仪观察SGC-7901细胞的凋亡情况;real time PCR法和Western blot法检测SGC-7901细胞Livin和Caspase-3 mRNA和蛋白的表达情况?结果:PDTC作用不同时间后对SGC-7901细胞生长有抑制作用,并诱导SGC-7901细胞凋亡,呈剂量-时间依赖性;PDTC可降低Livin mRNA及蛋白的表达,增加Caspase-3 mRNA及蛋白的表达,且Livin的表达量与PDTC的作用呈剂量,时间负相关?结论:PDTC可诱导人胃癌细胞株SGC-7901凋亡,其机制可能与PDTC抑制NF-κB信号转导通路,下调Livin表达继而上调Caspase-3表达有关?
英文摘要:
       Objective:To detect the effect of PDTC,the selective inhibitor of NF-κB,on the growth and apoptosis of human gastric carcinoma cell SGC-7901,to analyze the expression of Livin and Caspase-3 of cell SGC-7901treated by PDTC,and to explore the mechanism of PDTC in induction of tumor cell?蒺s apoptosis. Methods:After the treatment with PDTC on human gastric carcinoma cell SGC-7901,MTT assay was used to observe the growth inhibition of cell SGC-7901.The cell apoptosis was assessed by flow cytometry (FCM). The mRNA and protein expressions of Livin and Caspase-3 were detected by real time PCR and Western blot assay,respectively. Results:When PDTC was given,growth inhibition and elevated apoptosis of SGC-7901 cells were detected,which was showed in a dose- and time- dependent manner. PDTC also down-regulated the level of mRNA and protein expression of Livin,and elevated the mRNA and protein expression of Capase-3. Conclusion:PDTC can induce apoptosis of human gastric carcinoma cell SGC-7901 cells,which may be performed by down-regulating of Livin and up-regulating of Caspase-3 expression.
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