文章摘要
何帮顺,潘玉琴,瞿 卫,立 彦,王自正,王书奎.化疗药物对高表达SNCG的乳腺癌细胞株T47D的抑制作用的实验研究[J].南京医科大学学报,2007,(8):821~824
化疗药物对高表达SNCG的乳腺癌细胞株T47D的抑制作用的实验研究
Experimental research on the inhibition of chemotherapeutic drugs to the breast cancer cell line T47D with high expression of SNCG
投稿时间:2006-11-13  
DOI:10.7655
中文关键词: γ突触核蛋白  乳腺癌  T47D细胞  个体化治疗
英文关键词: γ-synuclein  breast carcinoma  T47D cell line  individual therapy
基金项目:南京市科技局科技发展指导性计划基金资助项目(2005指导0584)
作者单位
何帮顺 南京师范大学生命科学学院,江苏 南京 210029 
潘玉琴 南京师范大学生命科学学院,江苏 南京 210029 
瞿 卫 南京医科大学附属南京第一医院中心实验室,江苏 南京 210006 
立 彦 南京医科大学附属南京第一医院中心实验室,江苏 南京 210006 
王自正 南京医科大学附属南京第一医院中心实验室,江苏 南京 210006 
王书奎 南京医科大学附属南京第一医院中心实验室,江苏 南京 210006 
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中文摘要:
      目的:探讨各种化疗药物对高表达γ突触核蛋白(SNCG)的乳腺癌T47D细胞株的抑制作用?方法:常规培养不表达及高表达SNCG的乳腺癌MCF-7及T47D细胞株,采用MTT法观察顺铂(DDP)?阿霉素(ADM)?5-氟尿嘧啶(5-FU)?长春新碱(VCR)?紫杉醇(TAX)?依立替康(CPT-11)对细胞的抑制作用,并用流式细胞术分析药物作用后的细胞周期变化及细胞凋亡?结果:作用60 h后,ADM?VCR?TAX及CPT-11对T47D细胞的抑制作用显著低于MCF-7细胞(P < 0.01),而DDP?5-FU对T47D细胞的抑制作用与MCF-7细胞相比差异无显著性(P > 0.05),且显著高于其他4种抗肿瘤药物(P < 0.01)?药物作用T47D细胞60 h后,DDP?5-FU组的细胞增殖指数(PI)及Caspase-3表达均较对照组及其他4组差异有极显著性(P < 0.01)?结论:DDP及5-FU对高表达SNCG的T47D细胞的抑制作用显著高于ADM?VCR?TAX及CPT-11,此可为临床对乳腺癌患者个体化治疗方案的选择提供体外实验依据?
英文摘要:
      Objective:To explore the inhibition of different chemotherapeutic drugs to the breast cancer T47D cell line which highly expresses γ-synuclein(SNCG). Methods:T47D with SNCG expression and MCF-7 without SNCG expression cell lines were routinely cultured. MTT method was employed to evaluate the inhibiting roles of 6 chemotherapeutic drugs(DDP,ADM,5-FU,VCR,TAX and CPT-11) to the two cell lines. Moreover, Flow cytometry was enrolled to analyze the cell cycles and the apoptosis of T47D cell lines. Results:The inhibiting roles of ADM,VCR,TAX and CPT-11 to T47D were much lower than that of MCF-7 cell line after 60 h treatment(P < 0.01), while the effects of 5-FU and DDP to T47D cell line were not only the same as to MCF-7, but also much higher than the other 4 drugs(P < 0.01). Moreover, there were significant differences of PI and caspase-3 expressions of T47D cell line between DDP,5-FU groups and control or other 4 groups, respectively(P < 0.01). Conclusion:The inhibiting effects of DDP,5-FU to T47D cell line with high expression of SNCG are much higher than those of ADM,VCR,TAX and CPT-11,which can provide experimental evidence in vitro for the chemotherapy strategy to breast cancer patients.
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