文章摘要
杨国仪,陈静瑜,夏钰弘,洪建刚,虞敏红,吴 波,姜少军,周晓军.棉子糖低钾右旋糖酐液对猪供体肺灌注保护作用的病理学研究[J].南京医科大学学报,2007,(8):860~863
棉子糖低钾右旋糖酐液对猪供体肺灌注保护作用的病理学研究
A pathologiacal study of pig lung donor perfused with raffinose-low potassium dextran sol-ution for lung transplantation
投稿时间:2006-11-05  
DOI:10.7655
中文关键词: 肺移植  供体  保存  病理学  免疫组织化学  电子显微镜
英文关键词: lung transplantation  donor  preservation  pathology  immunohistochemistry  electronic microscope
基金项目:
作者单位
杨国仪 无锡市胸科医院肺移植中心病理科,江苏 无锡 214073 
陈静瑜 无锡市胸科医院肺移植中心病理科,江苏 无锡 214073 
夏钰弘 无锡市胸科医院肺移植中心病理科,江苏 无锡 214073 
洪建刚 无锡市胸科医院肺移植中心病理科,江苏 无锡 214073 
虞敏红 无锡市胸科医院肺移植中心病理科,江苏 无锡 214073 
吴 波 南京军区南京总医院病理科,江苏 南京 210002 
姜少军 南京军区南京总医院病理科,江苏 南京 210002 
周晓军 南京军区南京总医院病理科,江苏 南京 210002 
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中文摘要:
       目的:探讨棉子糖低钾右旋糖酐液(raffinose-low-potassium dextran solution,R-LPD液)供肺灌注保存后对肺组织形态学变化的影响?方法:应用光镜?电镜和免疫组化技术对低钾右旋糖酐液(low-potassium dextran solution,LPD 液)和R-LPD 液灌注保存的各10 例猪供肺组织按不同的保存时间段进行对比观察?结果:随着保存时间的延长,供肺的形态结构改变也逐渐明显,保存24 h后,LPD 液灌注组肺泡间隔断裂,肺泡上皮细胞变性?坏死?脱落,肺泡壁及血管周围间质组织水肿;而R-LPD 液灌注组形态改变则相对较轻?结论:R-LPD 液与LPD 液相比,在供肺灌注保存中更能起到保护肺泡上皮细胞的作用?
英文摘要:
      Objective:To observe the change of the morphological structure in lung perfused with raffinose-low-potassium dextran solution(R-LPD?蒺S). Methods:The 20 pig donor lung perfused with low-potassium dextran solution(LPD?蒺S) and R-LPD?蒺S was observed by light microscope, electronic microscope and immunohistochemistry. Results:Morphologic changes of the structure in the tissue cell were gradually obvious along with time. After 24 h of preservation, the tissue of donor lung perfused with LPD?蒺S showed a decreased number of intact type Ⅱ pneumocytes and significant cellular necrosis. Interstitial and alveolar edema with interstitial macrophage infiltration was also observed. Alveolar capillaries were collapsed. In contrast, R-LPD’S lungs showed only mild alterations such as minor interstitial edematous expansion, fewer damaged cells, and minor capillary injury. Conclusion:R-LPD’S exerts a cytoprotective effect on preservation of donor lung during pulmonary grafts, and it should be superior to LPD-S for this purpose.
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