文章摘要
蒋国军,王洪敏,周 炎,谈永飞,丁伟良,高 君,柯 巧,王 艳,沈 靖,徐耀初,沈洪兵.环氧化酶-2基因多态性与胃癌易感性的关联研究[J].南京医科大学学报,2007,(8):890~894
环氧化酶-2基因多态性与胃癌易感性的关联研究
The correlation study between the nucleotide polymorphisms of cyclooxygenase-2 gene and the susceptibility to gastric cancer
投稿时间:2006-12-22  
DOI:10.7655
中文关键词: 胃癌  COX-2基因  单核苷酸多态性  遗传易感性
英文关键词: gastric cancer  cyclooxygenase-2  single nucleotide polymorphisms  susceptibility
基金项目:国家自然科学基金资助项目(30271148和30571605)和江苏省社会发展资助项目(BS2004510)
作者单位
蒋国军 宜兴市人民医院肿瘤科,江苏 宜兴 214200 
王洪敏 宜兴市人民医院肿瘤科,江苏 宜兴 214200 
周 炎 宜兴市人民医院肿瘤科,江苏 宜兴 214200 
谈永飞 宜兴市人民医院肿瘤科,江苏 宜兴 214200 
丁伟良 宜兴市人民医院肿瘤科,江苏 宜兴 214200 
高 君 南京医科大学流行病与卫生统计学系,江苏 南京 210029 
柯 巧 南京医科大学流行病与卫生统计学系,江苏 南京 210029 
王 艳 南京医科大学流行病与卫生统计学系,江苏 南京 210029 
沈 靖 南京医科大学流行病与卫生统计学系,江苏 南京 210029 
徐耀初 南京医科大学流行病与卫生统计学系,江苏 南京 210029 
沈洪兵 南京医科大学流行病与卫生统计学系,江苏 南京 210029 
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中文摘要:
       目的:探讨环氧化酶-2基因(COX-2)的两个功能性单核苷酸多态(Single nucleotide polymorphisms,SNPs)与胃癌易感性的关系?方法:采用病例-对照研究设计,研究对象为254例经组织学确诊的胃癌病例和304例按年龄?性别进行匹配的无肿瘤史的社区对照人群?采用聚合酶链反应-限制性片段长度多态性方法(PCR-RFLP)测定COX-2基因-1195G/A多态,错配聚合酶链反应-限制性片段长度多态性方法(PIRA-PCR)测定COX-2基因 8473T/C多态的基因型,比较不同基因型与胃癌风险的关系,并探讨不同环境因素在其中所起的影响?结果:COX-2-1195GG?GA和AA三种基因型以及8473TT?TC和CC三种基因型在病例组和对照组间的频率分布无统计学意义?Logistic回归分析也未发现COX-2 SNP-1195G/A以及SNP8473T/C与胃癌的风险度关联有统计学意义(SNP-1195 GA+AA:校正OR=1.09,95%CI=0.70-1.67;SNP8473 TC+CC:校正OR=1.18,95%CI = 0.83-1.69)?结论:COX-2-1195G/A和8473T/C多态性可能与胃癌易感性无关,尚需进一步大样本量的研究予以证实?
英文摘要:
       Objective:To explore the correlation of two functional nucleotid epolymorphisms in the cyclooxygenase-2(COX-2) gene with susceptibitity to gastric cancer in a Chinese population of Yixing city. Methods:Polymerase chain reaction-restricted fragments length polymorphism(PCR-RFLP) and the primer-introduced restriction analysis(PIRA-PCR) assay was used to defect genotype of the COX-2 -1195G/A and 8473T/C polymorphisms, respectively. In a case-control study, 254 gastric cancer cases and 304 cancer-free controls were included in a Chinese population of Yixing city. Results:There was no statistical difference in the genotype frequencies of these two polymorphisms between the cases and controls(P = 0.695 for -1195G/A and P = 0.762 for 8473T/C). Logistic regression analyses showed that both variant genotypes of COX-2 -1195 and COX-2 8473 polymorphisms were not significantly associated with susceptibility to gastric cancer(adjusted OR=1.09,95% CI=0.70-1.67 for SNP -1195 GA+AA and adjusted OR=1.18,95% CI=0.83-1.69 for SNP8473 TC+CC). Conclusion:These findings suggested that these two COX-2 polymorphisms may not be associated with genetic susceptibility to gastric cancer.
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