文章摘要
董汉章,杨 力,夏建国,陈国玉.Survivin在胃癌SGC7901顺铂耐药中的作用[J].南京医科大学学报,2007,(9):927~930
Survivin在胃癌SGC7901顺铂耐药中的作用
The role of Survivin in SGC7901 resistance to cisplatin
投稿时间:2007-04-19  
DOI:10.7655
中文关键词: 胃癌  耐药  顺铂  Survivin
英文关键词: gastric carcinoma  drug resistance  cisplatin  Survivin
基金项目:
作者单位
董汉章 南京医科大学第一附属医院普外科,江苏 南京 210029 
杨 力  
夏建国  
陈国玉  
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中文摘要:
      目的:探讨Survivin在小剂量顺铂长期作用于SGC7901细胞导致细胞耐药中的作用机制?方法:体外诱导建立人胃癌顺铂耐药细胞株SGC7901/CDDP,显微镜下观察SGC7901/CDDP与正常SGC7901细胞株的形态差异,应用RT-PCR技术及Western blot技术分别检测SGC7901/CDDP与正常SGC7901细胞株中Survivin mRNA的表达和Survivin 蛋白的表达?结果:SGC7901/CDDP与正常SGC7901细胞株有明显的形态差异; SGC7901/CDDP中Survivin mRNA的表达和Survivin蛋白的表达都高于SGC7901细胞?结论:SGC7901对顺铂耐药可能与顺铂诱导SGC7901/CDDP中Survivin表达增加有关?
英文摘要:
      Objective:To study the mechanism on SGC7901 resistance to the low dose cisplatin. Methods:SGC7901/CDDP was established in vitro. The morphology change between SGC7901/CDDP and SGC7901 was observed by microscope. The mRNA and protein expression of Survivin in SGC7901/CDDP and SGC7901 were detected by RT-PCR and Western-blot,respectively. Results:There were morphological differences between SGC7901 and SGC7901/CDDP. The mRNA and protein expressions of Survivin in SGC7901/CDDP were both higher than in SGC7901. Conclusion:SGC7901 resistance to cisplatin may have association with the increased expression level of Survivin in SGC7901/CDDP.
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