文章摘要
李 蓓,孙卫斌,王 娟,徐 艳.硝苯地平对牙龈成纤维细胞Ⅰ型胶原合成的影响[J].南京医科大学学报,2007,(9):960~963
硝苯地平对牙龈成纤维细胞Ⅰ型胶原合成的影响
The effect of nifedipine on the expression of type I collagen in gingival fibroblasts
投稿时间:2007-04-20  
DOI:10.7655
中文关键词: 成纤维细胞  龈增生  Ⅰ型胶原  硝苯地平
英文关键词: fibroblast  gingival hyperplasia  type I collagen  nifedipine
基金项目:江苏省自然科学基金资助(BJ99068)
作者单位
李 蓓 南京医科大学口腔医学研究所,南京医科大学附属口腔医院口腔内科,江苏 南京 210029 
孙卫斌  
王 娟  
徐 艳  
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中文摘要:
      目的:探讨硝苯地平对人牙龈成纤维细胞Ⅰ型胶原合成的影响?方法:用含浓度为108?360?1 200 μg/L硝苯地平的DMEM液作用体外原代培养人牙龈成纤维细胞;酶联免疫吸附试验检测细胞Ⅰ型胶原表达水平;细胞计数观察细胞增殖?结果:Ⅰ型胶原合成检测和细胞增殖试验的结果都显示在第5天,高浓度1 200 μg/L?360 μg/L组与低浓度108 μg/L之间存在显著性差异?结论:高浓度硝苯地平有促进人牙龈成纤维细胞Ⅰ型胶原合成和细胞增殖的作用?
英文摘要:
      Objective:To investigate the effect of nifedipine,a calcium channel blocker,on the expression of collagen in gingival fibroblasts in vitro. Methods:Primarily gingival fibroblasts were cultured and incubated with various concentrations of nifedipine (108?360?1 200 μg/L). Enzyme-linked immunosorbent assay was used to evaluate the amount of type I collagen. Cell proliferation was measured by cell counting. Results:The expressions of collagen and cell proliferation were significantly different among the high concentration groups and the others on 5 th day,especially higher in 360 μg/L and 1 200 μg/L group. Conclusion: Nifedipine can increase the expression of type Ⅰ collagen in gingival fibroblasts and cell proliferation.
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