文章摘要
马艳萍,朱承华,杭 荟.氨基胍对大鼠急性高眼压视网膜一氧化氮合酶分布的影响[J].南京医科大学学报,2007,(9):977~980
氨基胍对大鼠急性高眼压视网膜一氧化氮合酶分布的影响
The effect of aminoguanidine on the level of nitric oxide synthase on retina in high ocular pressure rat models
投稿时间:2007-02-10  
DOI:10.7655
中文关键词: 氨基胍  一氧化氮合酶  高眼压  视网膜
英文关键词: AG  NOS  intraocular hypertension  retina
基金项目:
作者单位
马艳萍 南京医科大学第一附属医院眼科,江苏 南京 210029 
朱承华  
杭 荟  
摘要点击次数: 914
全文下载次数: 120
中文摘要:
      目的:观察大鼠急性高眼压模型中视网膜一氧化氮合酶(nitric oxidesynthase,NOS)分布及含量的变化,探讨氨基胍(AG)在高眼压诱导的视神经损伤中的神经保护作用?方法:SD大鼠27只,随机选取3只作为空白对照组(6眼),余24只施行高压前房灌注,眼压升高60 min后,随机取12只腹膜腔内注射氨基胍溶液(实验Ⅱ组),另外12只腹膜腔内注射等量生理盐水(实验Ⅰ组),再分别按高眼压术后时间不同随机分为4组即12 h组?24 h组?3天组和7天组,每组3只6眼;各组应用NADPH-d(还原型尼克酰胺嘌呤二核苷酸磷酸-黄递酶)法检测视网膜NOS的表达及分布情况?结果:空白对照组及实验组各组视网膜均有NOS表达,NOS阳性细胞在视网膜主要分布于视网膜节细胞层(ganglion cell layer, GCL)?内丛状层(inner plexiform layer ,IPL)?内核层(inner nuclear layer,INL); 实验Ⅰ组视网膜GCL的NOS阳性细胞数明显高于空白对照组(P < 0.01),实验Ⅱ组视网膜GCL的NOS阳性细胞数与实验Ⅰ组对照减少差异显著(P < 0.01)?结论:一氧化氮(NO)参与了高眼压诱导的视神经损伤; 氨基胍可能通过对NOS的抑制在大鼠高眼压诱导视网膜损伤中起到视神经保护作用?
英文摘要:
      Objective:To observe the nitric oxide synthase(NOS) expression in retina, and to investigate the function of the NOS inhibitor aminoguanidine(AG) in rats with acute intraocular hypertension. Methods:Twenty-seven SD rats were randomly divided into three groups:blank control(unoperational group),experimental group Ⅰ(ocular hypertension group),experimental group Ⅱ(drug-given group). Then each experimental group was divided into subgroups at 12 hours,24 hours,3 days and 7 days after perfusion,and NOS expression in the retina were detected by NADPH-diaphorase histochemistry. Results:The staining of NOS were localized in retinas of all groups. The count of NOS staining cells in the experimental group Ⅰ was more significant than that in the blank control. After AG was injected, the levels of NOS decreased. Conclusions:NO should play some cytotoxicity roles to retina. By inhibiting the activity of iNOS,AG might protect the retina against acute ocular hypertension injury.
查看全文   查看/发表评论  下载PDF阅读器
关闭