文章摘要
罗素菊,郑 焱,彭振辉,徐浩翔,张磐谏.他扎罗汀和窄谱中波紫外线联合对角质形成细胞他扎罗汀诱导基因3 mRNA的影响[J].南京医科大学学报,2007,(10):1067~1071
他扎罗汀和窄谱中波紫外线联合对角质形成细胞他扎罗汀诱导基因3 mRNA的影响
Synergistic effects of narrow-band UVB combined with tazarotene on inducing tazarotene-induced gene 3 mRNA level in normal human keratinocytes
投稿时间:2007-04-12  
DOI:10.7655
中文关键词: 角质形成细胞  他扎罗汀  维A酸  中波紫外线
英文关键词: keratinocyte  retinoid  tazarotene  UVB
基金项目:国家自然科学基金资助(NO 30371295和NO 30400387);陕西省自然科学基金资助(NO 2004C211)
作者单位
罗素菊 西安交通大学第二医院皮肤科,陕西 西安 710004 
郑 焱  
彭振辉  
徐浩翔  
张磐谏  
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中文摘要:
      目的:研究他扎罗汀和窄谱中波紫外线(NB-UVB)对培养的正常人角质形成细胞增殖和他扎罗汀诱导基因3(TIG3)mRNA表达的影响?方法:用0.1~1.0 ?滋mol/L他扎罗汀和/或50~100 mJ/cm2 NB-UVB处理正常人角质形成细胞24 h后,用MTT法和实时荧光定量RT-PCR法分别检测细胞增殖和TIG3 mRNA水平的改变?结果:0.1~1.0 ?滋mol/L他扎罗汀处理角质形成细胞后,可抑制细胞的增殖和上调TIG3 mRNA的水平,且较高剂量(1 μmol/L)的他扎罗汀的作用强于较低剂量(0.1 μmol/L)的他扎罗汀;NB-UVB单独作用时,未能明显抑制角质形成细胞的增殖和上调TIG3 mRNA的水平;二者联合作用时,对角质形成细胞的增殖抑制作用和TIG3 mRNA的诱导作用更强,且强于二者单独作用?结论:他扎罗汀单独处理可抑制角质形成细胞(KC)的增殖和上调TIG3 mRNA水平,但NB-UVB单独照射无此作用;二者联合作用时可协同抑制角质形成细胞的增殖和上调TIG3 mRNA的水平?
英文摘要:
       Objective:To investigate the alteration of cell proliferation and tazarotene-induced gene 3(TIG3) mRNA level in cultured normal human keratinocytes after tazarotene and narrow-band UVB(NB-UVB) combined treatment or alone. Methods:Normal human keratinocytes were exposed to NB-UVB(50-100 mJ/cm2) and/or followed incubation with tazarotene(0.1-1 μmol/L) for 24 hours,then the proliferation potency of the cells was detected by methyl thiazoleterazolium colorimetric assay and the level of TIG3 mRNA was examined by real-time quantitative reverse transcription polymerase chain reaction(RT-PCR). Results:Cultured keratinocytes proliferation was inhibited and TIG3 mRNA level was enhanced by both two doses of tazarotene single treatment but not by NB-UVB irradiation alone. Further,the higher dose of tazarotene showed more effects on inhibiting keratinocyte proliferation and elevating TIG3 mRNA level. Tazarotene-NB-UVB combination showed great effects on inhibiting keratinocyte proliferation and increasing TIG3 mRNA level,which were stronger than those induced separately. Conclusion:Tazarotene incubation single can inhibit keratinocyte proliferation and enhance TIG3 mRNA level,but NB-UVB irradiation alone can’t. Tazarotene-NB-UVB combination showed synergistic effects on inhibiting keratinocyte proliferation and increasing TIG3 mRNA level.
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