文章摘要
王卫文,张 戎,杨晓帆,夏永祥,李有武,王慧娟,季晓辉.正常人外周血中CD8+CD25+T细胞亚群的数量及其细胞因子表达的初步研究[J].南京医科大学学报,2007,(10):1092~1097
正常人外周血中CD8+CD25+T细胞亚群的数量及其细胞因子表达的初步研究
The preliminary study on the numbers and cytokine expression of CD8+CD25+ T cells from human peripheral blood
投稿时间:2007-04-26  
DOI:10.7655
中文关键词: 调节性T细胞  CD8+CD25+细胞亚群  TGF-β  IL-10  IFN-
英文关键词: regulatory T cell  CD8+CD25+ T cell  TGF-β  IL-10  IFN-γ
基金项目:南京医科大学科技发展重点项目(NY02069)
作者单位
王卫文 南京医科大学微生物学与免疫学系,江苏 南京 210029 
张 戎 南京医科大学微生物学与免疫学系,江苏 南京 210029 
杨晓帆 南京医科大学微生物学与免疫学系,江苏 南京 210029 
夏永祥 南京医科大学附属南京第一医院检验科,江苏 南京 210006 
李有武 南京医科大学附属南京第一医院检验科,江苏 南京 210006 
王慧娟 南京医科大学微生物学与免疫学系,江苏 南京 210029 
季晓辉 南京医科大学微生物学与免疫学系,江苏 南京 210029 
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中文摘要:
      目的:研究正常人外周血CD8+CD25+T细胞亚群的数量及其表达IFN-γ?IL-10和TGF-β的细胞数量情况,以期得到一组该亚群细胞正常数值范围,为研究CD8+CD25+T细胞亚群在自身免疫性疾病?过敏性疾病和器官移植中的变化及其临床意义提供参考?方法:分离正常人外周血单个核细胞,利用三色流式细胞术分别检测CD3+T细胞中CD8+CD25+?CD8+CD25-?CD8-CD25+和CD8-CD25-亚群的比例,并计算其细胞数量;并对经过或未经过离子霉素(ionomycin)?佛波醇乙酯(PMA)联合刺激5 h的CD8+CD25+T细胞检测表达IFN-γ?IL-10 及TGF-β的细胞数量情况?结果:正常人外周血CD3+T细胞中CD8+CD25+细胞亚群约为2.52%,数量达37.09 × 106/L,经离子霉素?佛波醇乙酯联合刺激培养5 h后其比例?数量均略有降低;CD8+CD25+T细胞表面TGF-β阳性率达到93.8%,刺激对其表达无明显影响;但刺激后IFN-γ+T细胞明显增多,而IL-10+的T细胞未见明显升高?结论:正常人外周血CD3+T细胞中存在少量的CD8+CD25+细胞亚群,该细胞表面稳定地高表达TGF-β,可对刺激呈现IFN-γ反应,但不对刺激产生增殖反应?
英文摘要:
      Objective:In order to know the clinical significance of the CD8+CD25+ T cell in autoimmune disease,hypersensitivity disease and organ transplantation, the normal limits of this T cell subset was obtained by studying the number of CD8+CD25+ T cell and its productions of IFN-γ,IL-10 and TGF-β in the normal peripheral blood. Methods:Mononuclear cells were isolated from healthy donors. The percentages and absolute numbers of CD8+CD25+,CD8+CD25-,CD8-CD25+ and CD8-CD25- from CD3+ T cells were detected by flow cytometry. In addition,stimulating with/without ionomycin and PMA 5 hours later,the expressions of IFN-γ,IL-10 and TGF-β in the CD8+CD25+ T cells was measured. Results:The percentage of CD8+CD25+ subgroup in CD3+ T cells from normal human peripheral blood was 2.52%,the absolute number was 37.09×106/L. The percentage and absolute number decreased after stimulated with ionomycin and PMA 5 hours. The expression of TGF-β on the surface of CD8+CD25+ T cells up to 93.8%,stimulation seems not obviously affected its expression but IFN-γ+ T cells increased obviously,however,IL-10+ T cells didn’t obviously up-regulat. Conclusion:There were a few CD8+CD25+ T cells in CD3+ T cells from normal peripheral blood. They expressed highly TGF-β in the cell surface,and could have IFN-γ reaction but no proliferation after stimulation.
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