文章摘要
洪 琴,池 霞,陈荣华,郭锡熔,费 莉,郭 梅,潘晓勤.MOBP基因在注意缺陷多动障碍大鼠前额叶皮层表达的研究[J].南京医科大学学报,2007,(10):1123~
MOBP基因在注意缺陷多动障碍大鼠前额叶皮层表达的研究
Expression of MOBP in the PFC of Animal Model of ADHD in SHR
投稿时间:2007-01-23  
DOI:10.7655
中文关键词: 注意缺陷多动症  MOBP  RT-PCR  电镜
英文关键词: attention deficit hyperactivity disorder  (ADHD)  myelin-associated oligodendrocytic basic protein(MOBP)  RT-PCR  electron microscope
基金项目:江苏省自然科学基金资助(BK2006009)
作者单位
洪 琴 南京医科大学儿科医学研究所,江苏 南京 210029 
池 霞 南京医科大学附属南京市妇幼保健院,江苏 南京 210004 
陈荣华 南京医科大学儿科医学研究所,江苏 南京 210029 
郭锡熔 南京医科大学儿科医学研究所,江苏 南京 210029 
费 莉 南京医科大学儿科医学研究所,江苏 南京 210029 
郭 梅 南京医科大学儿科医学研究所,江苏 南京 210029 
潘晓勤 南京医科大学儿科医学研究所,江苏 南京 210029 
摘要点击次数: 1404
全文下载次数: 83
中文摘要:
      目的:探讨注意缺陷多动障碍(ADHD)动物模型SHR大鼠前额叶皮层MOBP基因表达情况,分析其在ADHD病理生理学中的意义?方法:应用RT-PCR?免疫组化?电镜技术检测该基因mRNA?蛋白表达情况以及轴突髓鞘形态变化?结果:RT-PCR结果显示SHR大鼠脑组织中MOBP mRNA表达水平(35.78 ± 6.04)%明显低于正常对照WKY大鼠(69.41 ± 7.43)%(P < 0.01);免疫组化结果提示SHR大鼠前额叶皮层髓鞘着色显著变浅?稀疏,着色面积占总面积比例(7.43 ± 3.51)%较对照组WKY(14.39 ± 5.82)%明显减少(P < 0.05);电镜观察结果显示,部分SHR大鼠髓鞘放射性结构呈直线性分布?结论:MOBP基因在SHR中的表达下调可能通过参与损害中枢神经系统髓鞘结构和功能,在ADHD病理机制中发挥作用?
英文摘要:
      Objective:To explore the changes of the myelin-associated oligodendrocytic basic protein(MOBP) gene mRNA and protein expression in the prefrontal cortex(PFC) of SHR/WKY and to analyze the relationship between the MOBP and the etiology and pathophysiology of Attention Deficit Hyperactivity Disorder(ADHD). Methods:The expressive levels of MOBP in the PFC of SHR and WKY were verified by using RT-PCR technique. The distribution of the MOBP in rat brains was marked by used MOBP-HRP immunohistochemistry(IHC) and the electron microscope were applied as the ultrastructural study on brain tissues. Results:The levels of MOBP gene mRNA expression(35.78 ± 6.04)% in the PFC of SHR were significantly lower than those(69.41 ± 7.43)% in the PFC of WKY rats. At the same time,there was ultrastructural difference in arrangement of the radial component in myelin between two groups. Conclusion:The less of MOBP expression in the SHR may be one of the important pathophysiological bases for ADHD.
查看全文   查看/发表评论  下载PDF阅读器