文章摘要
王克俊,陈小平,戚美芬,张京平,徐 皓,纪俊标,朱春富.Lipo-PGE1对肠道瘀血再灌注微循环的干预作用[J].南京医科大学学报,2007,(11):1248~1251
Lipo-PGE1对肠道瘀血再灌注微循环的干预作用
Effects of Lipo-PGE1 on intestinal microcirculation during acute intestinal venous congestion reperfusion in rats
投稿时间:2007-06-06  
DOI:10.7655
中文关键词: Lipo-PGE1  瘀血再灌注  损伤  微循环
英文关键词: Lipo-PGE1  congestion and reperfusion  injury  microcirculation
基金项目:南京医科大学科技发展基金资助(NY04053)
作者单位
王克俊 南京医科大学附属常州二院普外科江苏 常州 213003 
陈小平 南京医科大学附属常州二院普外科江苏 常州 213003 
戚美芬 南京医科大学附属常州二院普外科江苏 常州 213003 
张京平 南京医科大学附属常州二院普外科江苏 常州 213003 
徐 皓 南京医科大学第一附属医院普外科江苏 南京 210029 
纪俊标 南京医科大学第一附属医院普外科江苏 南京 210029 
朱春富 南京医科大学附属常州二院普外科江苏 常州 213003 
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中文摘要:
      目的:研究Lipo-PGE1对肠道瘀血后再灌注微循环的干预作用。方法:30只大鼠随机分为3组:瘀血再灌注组、Lipo-PGE1组和假手术组。通过阻断门静脉45 min、再灌注60 min制作小肠瘀血再灌注模型。Lipo-PGE1组在门静脉夹闭前10 min、夹闭后即刻和开放后10 min静脉注入Lipo-PGE1 0.2 μg,瘀血再灌注组以生理盐水代替,假手术组不夹闭门静脉。再灌注后60 min观察回肠肠系膜微循环开放情况和出血灶数,取回肠段作病理检查。结果:急性瘀血再灌注后,瘀血再灌注组肠系膜微循环开放率较假手术组明显下降、毛细血管周围出血灶数明显增多,Lipo-PGE1治疗组较瘀血再灌注组开放率明显升高,但毛细血管周围出血灶数两组间无显著差异。病理检查示瘀血再灌注组Chiu分级较假手术组明显升高,而用Lipo-PGE1组Chiu分级较瘀血再灌注组显著降低。结论:Lipo-PGE1的干预可以明显改善肠道瘀血再灌注后微循环血流状况,减轻肠道组织的损伤。
英文摘要:
       Objective:To investigate the effects of Lipo-PGE1 on the microcirculation changes of intestine during acute intestinal venous congestion reperfusion in rats. Methods:30 SD rats were divided into three groups,the acute intestinal venous congestion reperfusion group(CR group), the Lipo-PGE1 treatment group(Lipo-PGE1 group) and the sham operation group(SO group). To induce intestinal venous congestion model, portal vein was clamped for 45 min, then unclamped for 60 min. In Lipo-PGE1 group, 0.2 μg Lipo-PGE1 was infused via veins 10 min before,just at clamping portal vein and 10 min after reperfusion respectively,while in CR group normal saline was used instead of Lipo-PGE1. The small intestinal mesenteric area was selected for observation of microcirculation. The numbers of capillary vessels with or without blood flow were recorded as reperfusion rate. Hemorrhagic spots around the capillary were also recorded. Intestinal species were obtained for histological examination after reperfusion for 60 min. The intestine injury was studied with Chiu’s Grade classification. Results:In CR group,the microcirculation reperfusion rates were much lower and the numbers of hemorrhagic spots around the capillary were much more than those in SO group. In Lipo-PGE1 group,however,the microcirculation reperfusion rates were much higher than in CR group,while no significant difference was found in hemorrhagic spots between the two groups. In CR group,optical microscopy showed that the intestine mucosa lesion was worse than that in SO group by Chiu’s Grade standard. But compared with CR group, it showed that intestine mucosa injury lesion was attenuated in Lipo-PGE1 group. Conclusion:Lipo-PGE1 could effectively protect microcirculation and attenuate the injury of intestine caused by acute intestinal venous congestion.
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