Objective：To assess the correlation between the HBx gene integration in hepatocyte chromosome and HCC generation, investigate the difference of hepatitis B virus serum markers between patients with HCC and CHB, and to investigate the correlation between HBx gene integration and hepatitis B virus serum markers. Methods：Hepatocyte samples obtained from 19 patients with hepatocyte carcinoma or 14 subjects with chronic hepatitis B were prepared. The frequency of HBx gene integration was detected using biotin-marked HBx probe. Then the results were analyzed combining with the clinical data of hepatitis B virus serum markers.Results：①Among 19 patients with HCC， 13 subjects were found the signals of HBx gene hybridization in mitotic chromosome of tumour，which occupied 68.24% and was more than that in CHB（28.57%）（-字2 = 5.12，P < 0.05）. The HBx integration sites were 3.8 ± 1.36 in hepatocyte chromosome of HCC and 1.3 ± 0.36 in CHB，respectively（t = 3.5，P < 0.01）. ②The positive rate of HBeAg in serum was 80.0% in CHB，which is much higher than that in HCC（10.53%）. There was a significant difference（-字2 = 6.33，P < 0.01） between those two groups. ③There were no correlation between the frequency of HBx integration and the clinical hepatitis B virus serum marks either in HCC or in CHB. Conclusion：The frequency of HBx gene integration in HCC is higher than that in CHB. HBV reproducing in patients with CHB is more active than that with HCC.