文章摘要
刘 军,黄 野,吕天润,曹晓建,陈 骐.自制脂质体包裹神经生长因子通过血脑屏障的初步研究[J].南京医科大学学报,2008,28(1):24~27
自制脂质体包裹神经生长因子通过血脑屏障的初步研究
The preliminary research of NGF packed with liposome crossing the blood-brain barrier
投稿时间:2007-07-07  
DOI:10.7655
中文关键词: 脂质体  神经生长因子  血脑屏障  单光子发射计算机断层显像  靶向性
英文关键词: liposome  nerve growth factor  blood brain barrier  single photon emission computerized tomography  ability of target.
基金项目:国家自然科学基金资助(30070901);江苏省高校自然科学基金资助(05KJB320080)
作者单位
刘 军 南京医科大学第二附属医院骨科,江苏 南京 210011 
黄 野 南京医科大学第二附属医院骨科,江苏 南京 210011 
吕天润 南京医科大学第一附属医院骨科,江苏 南京 210029 
曹晓建 南京医科大学第一附属医院骨科,江苏 南京 210029 
陈 骐 沈阳药科大学药学院,辽宁 沈阳 110001 
摘要点击次数: 1540
全文下载次数: 218
中文摘要:
      目的:研究脂质体对神经生长因子(NGF)包裹后促进其通过血脑屏障(BBB)的作用?方法:将99mTc-NGF用脂质体包裹后,按照以下条件处理:将包裹含有4.0mCi 99mTc-NGF的脂质体A注射至新西兰兔体内,利用SPECT分析其在脑部分布的百分数,对照组以相同放射剂量的99mTc-NGF?99mTc-NGF-普通脂质体B处理用来考察脑组织靶向性;同时以0.05 mg/kg剂量将NGF脂质体A?单纯NGF?空白脂质体?生理盐水分别注入新西兰兔体内,利用ELISA法于30?60?90?120 min测定脑脊液中NGF的浓度?结果:自制注射用脂质体A组包裹的99mTc-NGF经SPECT显像,其在脑部的放射性计数较高,而99mTc-NGF组几乎全部从泌尿系统代谢?99mTc-NGF-普通脂质体B组基本被网状内皮系统吞噬后沉积于肝脏;ELISA检测证明脂质体包裹的NGF能够部分通过血脑屏障?结论:此自制NGF-脂质体A具有一定的脑组织靶向性,能够携带NGF通过BBB?
英文摘要:
      Objective:To research the penetration to blood brain barrier(BBB) after nerve growth factor(NGF) packed with liposome. Methods:The 4.0mCi of 99mTc-NGF packed with liposomeA,4.0mCi of 99mTc-NGF packed with liposomeB and free 99mTc-NGF were injected into the rabbit by the vein of ear border respectively. SPECT was used to detect the distribution of 99mTc-NGF. And then the ratio of radioactivity counts were calculated to describe the character of distribution in brain;The NGF concentration in CSF was detected by ELISA after the NGF was injected into the rabbit at 30,60,90,120 min. Results:The results of imaging with SPECT showed that 99mTc-NGF packed with liposomeA was aggregated in brain; while 99mTc-NGF was evacuated through urinary system. In addition,99mTc-NGF packed with liposomeB was aggregated in liver;The NGF concentration in CSF showed that the NGF packed with liposome could penetrate the BBB partly. Conclusion:The ratio of brain tissue signal intensity to body signal intensity of NGF packed with liposomeA obviously exceeded that of NGF and liposomeB from beginning to terminal. The NGF packed with liposomeA could help the NGF penetration the BBB.
查看全文   查看/发表评论  下载PDF阅读器
关闭