连云港西汉女尸肌肉组织镜下形态学观察和DNA检测的探究
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Observation of micromorphology and DNA examination of the muscular tissue from the female cadaver of Xi Han Dynasty discovered in Lianyungang
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    摘要:

    目的:对2002年于连云港出土的西汉女尸肌肉组织进行镜下形态学和遗传学研究。方法:取出土后立即被浸泡在10%福尔马林溶液中的西汉女尸肌肉组织,使用光学显微镜和电子显微镜观察其形态结构,使用DNA提取试剂盒提取该组织DNA并使用特异性引物进行PCR扩增。结果:古尸标本光镜和电镜下结构均紊乱,未提取到基因组DNA和线粒体DNA。结论:福尔马林固定法可能对古尸标本后续的电镜检测和分子生物学研究不利。

    Abstract:

    Objective:To study the micromorphology and genetics of the muscular tissue from the female cadaver of Xi Han Dynasty discovered in Lianyungang in 2002. Methods:Light microscope and electron microscope were used to observe micro morph of the muscular tissue from the female cadaver,which had been fixed in 10% formalin after being unearthed. Besides,DNA Extract kits were used to extract genome DNA from muscular tissue;PCR was used to amplify mitochondrial DNA sequence. Results:Observed by both light microscope and electron microscope,the morph of the muscular tissue from the ancient cadaver sample was in a great mess. Genomic DNA(including mitochondrial DNA) of the cadaver was not obtained by extraction and amplification,respectively. Conclusion:The ancient cadaver fixed by formalin might be disadvantageous of the electron microscope and molecular biology research.

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陈 芳,骆 莺,韩群颖,王建文,王林涛.连云港西汉女尸肌肉组织镜下形态学观察和DNA检测的探究[J].南京医科大学学报(自然科学版),2008,28(3):335-337345

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  • 收稿日期:2007-12-14
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