文章摘要
谢建军,贾瑞鹏,罗斐埜,朱佳庚.缺血预适应对大鼠肾移植早期组织学及IKKβ、NF-kB、TNF-αmRNA表达的影响[J].南京医科大学学报,2008,28(4):503~507
缺血预适应对大鼠肾移植早期组织学及IKKβ、NF-kB、TNF-αmRNA表达的影响
Early effects of ischemic preconditioning on expressions of IKKβ,NF-kB,TNF-α mRNA and changes in rat kidney allograft tissue
投稿时间:2007-09-30  
DOI:10.7655
中文关键词: 缺血预适应  肾移植  IKKβ  NF-kB  TNF-α
英文关键词: ischemic preconditioning  kidney transplantation  IKKβ  NF-kB  TNF-α
基金项目:医药生物技术国家重点实验室开放基金资助项目(XW200202)
作者单位
谢建军 南京医科大学附属南京第一医院泌尿外科江苏 南京 210006 
贾瑞鹏 南京医科大学附属南京第一医院泌尿外科江苏 南京 210006 
罗斐埜 东南大学临床医学院江苏 南京 210009 
朱佳庚 南京医科大学附属南京第一医院泌尿外科江苏 南京 210006 
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中文摘要:
      目的:探讨缺血预适应(IPC)对大鼠同种异体移植肾早期组织学及IKKβ、NF-kB、TNF-αmRNA表达的影响。方法:36只成年SD大鼠为供受体,并随机分为以下三组:假手术组(A),剖腹探查后关腹;普通移植组(B),同种原位肾移植;缺血预适应组(C),供肾15 min缺血10 min灌注预处理肾移植。移植术后24 h,检测各组Scr、BUN水平及移植肾组织损伤程度,RT-PCR技术分别检测TNF-α、ⅠkB 激酶β(IKKβ)及NF-kB P65亚基的转录水平。结果:肾移植后24 h,与B组相比,C组肾小管损伤程度明显减轻(肾小管损伤指数P < 0.05),TNF-αmRNA 表达亦明显减弱(P < 0.05);但B、C两组IKKβ及P65 mRNA表达无明显差异(P > 0.05)。B、C两组Scr、BUN水平较A组明显升高,但两组间未有明显差异(P > 0.05)。结论:15 min/10 min缺血预适应能够明显减轻大鼠移植肾早期缺血再灌注损伤,其保护作用可能与TNF-α表达抑制,TNF-α/IKKβ/NF-kB P65正反馈信号阻断有关。
英文摘要:
      Objective:To investigate the early effects of ischemic preconditioning(IPC) on expressions of IKKβ,NF-?资B,TNF-α mRNA and the pathological changes in rat kidney allograft tissue. Methods:Thirty-six male SD rats were served as donors and recipients. Animals were randomly divided into the following three groups:the sham-operated group(A), regular transplantation group (B),and treatment group(C). Animals in A group were subjected to exploratory laparotomy. B group received orthotopic homotransplantations. IPC in C group was administered with 15 minutes of ischemic followed by 10 minutes of reperfusion. The changes of renal function and the degree of ischemic reperfusion injury of kidney graft were observed 24 hours after renal transplantation. Expressions of TNF-α,IKKβ,NF-kB p65 subunit mRNA were assessed by RT-PCR,respectively. Results:The degree of renal graft tubules injury in C group was significantly lower than that in the B group(P < 0.01),as well as the transcription of TNF-α(vs non-IPC group P < 0.01) after 24 hours of transplant. No significant difference of the expression of IKKβ mRNA and p65 mRNA was found between C group and B group(P > 0.05). The levels of Scr and BUN were higher than that in A group, whereas no significant difference was found between C group and B group(P > 0.05). Conclusion:One cycle schedule of preconditioning attenuated rat renal allograft ischemia reperfusion injury in early phase. The inhibition of TNF-α expression and the positive feedback of TNF-α/NF-kB signaling pathways might play the role for the protection.
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