文章摘要
黄海慧,朱敏敏,张小宝,傅诚章.氯胺酮对哮喘模型大鼠氧化应激的影响[J].南京医科大学学报,2008,28(5):597~600688
氯胺酮对哮喘模型大鼠氧化应激的影响
Effects of Ketamine on oxidative stress in Brown-Norway rats with asthma
投稿时间:2007-08-29  
DOI:10.7655
中文关键词: 氯胺酮  支气管哮喘  氧化应激  活性氧族  p38
英文关键词: Ketamine  asthma  oxidative stress  ROS  p38
基金项目:江苏省自然科学基金资助项目(BK2001160)
作者单位
黄海慧 南京医科大学第一附属医院麻醉科,江苏 南京 210029 
朱敏敏  
张小宝  
傅诚章  
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中文摘要:
      目的:研究氯胺酮对哮喘大鼠氧化应激反应产物及相关蛋白表达?方法:采用鸡卵蛋白(OVA)辅以百日咳杆菌菌苗和氢氧化铝为佐剂注射致敏大鼠?雾化吸入OVA激发?51只大鼠随机分成对照组(C组)鸡卵蛋白组(A组),氯胺酮雾化吸入组(K组),氯胺酮腹腔注射组(V组)?C组采用PBS替代OVA进行雾化吸入?A组在激发前给予PBS雾化吸入,K1?K2?K3组大鼠在激发前分别给予12.5?25.0及50.0 mg/ml浓度的氯胺酮雾化吸入,V1?V2组在激发前分别给予50 μg/kg和100 μg/kg的氯胺酮腹腔注射?取大鼠血清?肺组织,分别测定样本中抗氧化应激能力及氧化应激致炎症下游产物P38蛋白的激活?结果:氯胺酮处理组抗OH·?O2·-能力所测得值均高于A组,其中K1?K2?V1组与A组比较差异有显著性(P < 0.01),K3?V2组与A组比较差异有显著性(P < 0.05)?氯胺酮处理组SOD活力明显高于A组,其中K1?K2?V1组与A组比较差异有显著性(P < 0.01),K3?V2组与A组比较差异有显著性(P < 0.05),与C组相比,A组的抗氧化应激能力均降低(P < 0.01)?与C组相比,A组p38的激活程度(pp38/p38)增高(P < 0.01)?p38的激活程度在氯胺酮处理组均降低于A组,其中K1?K2?V1组与A组比较(P < 0.01),K3?V2组与A组比较(P < 0.05)?结论:氯胺酮雾化吸入和腹腔注射可抑制卵蛋白所致的哮喘大鼠肺氧化应激反应的增高,并抑制p38蛋白的激活?雾化吸入氯胺酮12.5 mg/ml和腹腔注射50.0 μg/kg已达到治疗效果?
英文摘要:
      Objective:To investigate the effect of nebulized Ketamine inhalation and i.p. injection on oxidative stress in the lungs of asthmatic rats. Methods:Fifty-one Brown Norway rats(were randomly assigned to seven groups,which were control group(C) with 7 rats,asthma group(A) with 8 rats,Ketamine inhaled groups(K1,K2,K3) each with 8 rats,and Ketamine i.p. injected groups(V1?V2) each with 6 rats. In group A,K1,K2,K3,V1,V2,asthma was induced by two steps:The rats were received subcutaneous injection of ovalbumia and aluminum hydroxide in PBS,and then inhaled nebulized 1% OVA in PBS. In group K1,K2 and K3,the sensitized rats were exposed to 12.5 mg/ml(K1 group),25.0 mg/ml(K2 group),50.0 mg/ml(K3 group)of nebulized Ketamine before inhaling nebulized OVA. In groupV1 and V2,the sensitized rats were i.p. injected with 50 μg/kg ketamine(V1) and 100 μg/kg(V2) before inhaling nebulized OVA. Lung samples were taken and made into tissue bomogenate,and the protein were extracted. ROS were measured with kit,and p38 was detected by Western-blot. Results:The ability of anti OH· and O2·- of group A was lower than group K1?K2 and V1(P < 0.01)and K3?V2(P < 0.05). The activity of SOD of group A was lower than group K1?K2 and V1(P < 0.01) and K3?V2(P < 0.05). Compared with group C,the ability of anti-oxidative stress of group A was significantly decreased(P < 0.01). Compared with group C,the activation of p38 of group A was significantly increased(P < 0.01),also the activation of p38 in group A was higher than group K1?K2 and V1(P < 0.01) and K3?V2(P < 0.05). Conclusion:Inhalation of nebulized Ketamine and i.p. injected with Ketamine can restrain the oxidative stress reaction and the activation of p38 in lung in asthmatic rats. 12.5 mg/ml nebulized ketamine and 50.0 μg/kg i.p. injected with Ketamine appears to be enough for satisfactory clinical results.
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