文章摘要
汪 剑,卓莉莉,姜之文,龚 杰,盛燕辉,杨 荣,孔祥清,曹克将.CSX/Nkx2.5基因在大鼠胚胎心脏发育过程中的表达[J].南京医科大学学报,2008,28(5):605~608
CSX/Nkx2.5基因在大鼠胚胎心脏发育过程中的表达
The expression and location of CSX/Nkx2.5 in the developing rat hearts
投稿时间:2008-03-10  
DOI:10.7655
中文关键词: CSX/Nkx2.5基因  大鼠  胚胎  心脏
英文关键词: CSX/Nkx2.5 gene  rat  embryo  heart
基金项目:国家自然科学基金项目资助(30770892)
作者单位
汪 剑 南京医科大学第一附属医院心脏科,江苏 南京 210029 
卓莉莉  
姜之文  
龚 杰  
盛燕辉  
杨 荣  
孔祥清  
曹克将  
摘要点击次数: 1413
全文下载次数: 220
中文摘要:
      目的:检测CSX/Nkx2.5基因在大鼠胚胎心脏发育过程中的mRNA表达变化及其蛋白定位?方法:取大鼠胚胎d12?d15?d17?d19心脏,采用半定量反转录聚合酶链反应(RT-PCR)技术检测心脏组织中CSX/Nkx2.5基因mRNA的表达丰度,并应用免疫组化的方法对其进行定位分析?结果:在大鼠胚胎d12?d15?d17?d19心脏中,CSX/Nkx2.5 基因mRNA均有表达,且呈逐渐上调趋势;其蛋白主要表达于心房?心室?肌小梁等处心肌组织,而在心内膜?心外膜?瓣膜?流出道?大血管等处未见表达?结论:CSX/Nkx2.5 基因的表达随大鼠胚胎心脏的生长发育逐渐上调,且特异表达于胚胎心脏心肌细胞的细胞核,与心脏发育密切相关?
英文摘要:
      Objective:To investigate the change of CSX/Nkx2.5 gene mRNA expression and its protein location in the developing rat hearts. Methods:Embryonic hearts of normal rats were obtained on the 12th,15th,17th and 19th day during embryonic period. Reverse transcription polymerase chain reaction(RT-PCR) was used to reveal CSX/Nkx2.5 gene mRNA expression,and Immunohistochemistry for its protein location. The results were analyzed by the statistical method of One-way ANOVA. Results:With the development of rat hearts,the expression levels of CSX/Nkx2.5 gene mRNA were up-regulated. CSX/Nkx2.5 protein was mainly expressed in the atria ,ventricles and trabecular muscles,while it was not detected in the endocardium, epicardium, valvular tissues, outflow tracks and large arteries. Conclusion:CSX/Nkx2.5 gene is up-regulated in the rat heart and specifically expressed in the nucleus of cardiac muscle cell. It suggests that CSX/Nkx2.5 should play an important role in the fetal heart development.
查看全文   查看/发表评论  下载PDF阅读器
关闭