文章摘要
莫 萍,施瑞华,张红杰,张国新.膜联蛋白A10在人食管鳞癌及其癌旁组织中的表达及临床意义[J].南京医科大学学报,2008,28(5):658~661
膜联蛋白A10在人食管鳞癌及其癌旁组织中的表达及临床意义
Detection of annexin A10 in human esophageal squamous cancer tissues
  
DOI:10.7655
中文关键词: 膜联蛋白A10  人食管鳞癌  免疫印迹
英文关键词: annexin A10  Human esophageal squamous cell cancer  Western blot
基金项目:江苏省卫生厅重点医学人才培养项目(RC2007045)
作者单位
莫 萍 南京医科大学第一附属医院消化内科,江苏 南京 210029 
施瑞华  
张红杰  
张国新  
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中文摘要:
      目的:探讨膜联蛋白A10(annexin A10)在人食管鳞癌和癌旁组织中的表达以及临床意义?方法:应用半定量RT-PCR?定量PCR?Western blot法,检测40对配对人食管鳞癌和癌旁组织中annexin A10表达差异;并分析该表达差异与临床病理特征如性别?年龄?肿瘤大小?浸润深度?临床分期?淋巴结转移和细胞分化的关系?结果:40对配对人食管鳞癌组织中,annexin A10 mRNA表达水平癌组织77.06 ± 37.11,癌旁组织66.99 ± 17.62;蛋白表达水平癌组织为1.73 ± 1.30,癌旁组织1.26 ± 1.18,癌组织和配对癌旁组织表达差异有统计学意义(P < 0.05)?annexin A10在癌组织和癌旁组织的表达差异在淋巴结转移组和未转移组,在高中低分化组均有显著性差异,而与性别?年龄?肿瘤大小?浸润深度?临床分期关系无统计学意义?结论:annexin A10在人食管鳞癌中表达上调,并随肿瘤分化程度降低?淋巴结转移而表达增高?annexin A10可能成为食管鳞癌诊断和预后的一个新的指示靶点?
英文摘要:
      Objective:To investigate the expression of annexin A10(ANXA10) in human esophageal squamous cancer tissues,and to discuss its value in the development of human esophageal squamous cell cancer(SCC). Methods:The expression of annexin A10 in 40 matched-pairs of SCC tissues were detected by RT-PCR,Real-time PCR,Western blot methods. Results:The relative intensities of annexin A10 mRNA expression in SCC tissues and peri-carcinoma tissues were 77.06 ± 37.11,66.99 ± 17.62 respectively. The relative intensities of annexin A10 protein expression in SCC tissues and peri-carcinoma tissues were 1.73 ± 1.30,1.26 ± 1.18 respectively. There were remarkable differences between SCC and esophageal peri-carcinoma tissues. There were also remarkable differences between lymph node metastasis group and non-metastasis group; among well,moderately and poorly differentiated groups. Conclusion:The expression of annexin A10 in SCC is up-regulated and associated with cell differentiation and lymph-node metastasis. Detection of annexin A10 might be a valuable tumor marker and prognostic factor for SCC.
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