| 梁晓晖,平其能,刘 军,吕文莉,侯冬枝.肝靶向牛血清白蛋白脂质体的制备及理化性质研究[J].南京医科大学学报,2008,28(10):1229~1233 |
| 肝靶向牛血清白蛋白脂质体的制备及理化性质研究 |
| Studies on preparation of hepatic-targeted bovine serum albumin liposome and its physic-ochemical properties |
| 投稿时间：2008-01-29 |
| DOI：10.7655 |
| 中文关键词: 肝靶向脂质体 O-羧甲基-N-乳糖酰化壳聚糖 逆相蒸发法 包封率 |
| 英文关键词: hepatic-targeted liposome Gal-CMCS reverse evaporation method entrapment efficiency |
| 基金项目:江苏省教育厅高校自然基础研究基金资助(06KLB320075);南京医科大学自然科学基金资助(NY04030);国家博士后基金(2005037423) |
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| 中文摘要: |
| 目的:研制肝靶向牛血清白蛋白(bovine serum albumin,BSA)脂质体,并研究其理化性质,为蛋白质药物的肝靶向给药提供一种新模型?方法:首先采用逆相蒸发法制备BSA负电荷脂质体,再用O-羧甲基-N-乳糖酰化壳聚糖(O-carboxymethyl N-galactosylated chitosan,Gal-CMCS)修饰,制备肝靶向BSA脂质体,并考察脂质体的形态?粒径?Zeta 电位?包封率及稳定性等理化性质?结果:制得的肝靶向BSA脂质体在电镜下呈类圆形,粒径分布均匀,平均粒径为(284.1 ± 5.41)nm,BSA包封率为(34.21 ± 1.73)%,Zeta 电位为(+27.1 ± 1.17)mV,4℃下放置3个月粒径和包封率无明显变化?结论:成功制备了肝靶向BSA脂质体,其具有良好的稳定性和较高的药物包封率,可作为蛋白质药物肝靶向给药的一种新模型? |
| 英文摘要: |
| Objective:To prepare the hepatic-targeted Bovine serum albumin(BSA)liposome and study its physicochemical properties, to providing a new hepatic-targeted drug delivery system for protein-drug. Methods:Liposome was prepared by reverse phase evaporation method,and then the liposome was coated with O-carboxymethyl N-galactosylated chitosan(Gal-CMCS). The shape,particle size,Zeta potential,entrapment efficiency and stability were investigated. Results:Hepatic-targeted BSA liposome was round and uniform;its mean particle size was(284.1±5.41)nm;the entrapment efficiency was(34.21±1.73)%; Zeta potential was(+27.1±1.17) mV;and there was no obvious change in entrapment efficiency and particle size at 4℃ within 3 months. Conclusion:The hepatic-targeted BSA liposome was successfully prepared,with good stability and high entrapment efficiency,thus providing a new hepatic-targeted drug delivery system for protein-drug. |
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