文章摘要
梁晓晖,平其能,刘 军,吕文莉,侯冬枝.肝靶向牛血清白蛋白脂质体的制备及理化性质研究[J].南京医科大学学报,2008,28(10):1229~1233
肝靶向牛血清白蛋白脂质体的制备及理化性质研究
Studies on preparation of hepatic-targeted bovine serum albumin liposome and its physic-ochemical properties
投稿时间:2008-01-29  
DOI:10.7655
中文关键词: 肝靶向脂质体  O-羧甲基-N-乳糖酰化壳聚糖  逆相蒸发法  包封率
英文关键词: hepatic-targeted liposome  Gal-CMCS  reverse evaporation method  entrapment efficiency
基金项目:江苏省教育厅高校自然基础研究基金资助(06KLB320075);南京医科大学自然科学基金资助(NY04030);国家博士后基金(2005037423)
作者单位
梁晓晖 中国药科大学药学院,江苏 南京 210009 
平其能 中国药科大学药学院,江苏 南京 210009 
刘 军 南京医科大学第二附属医院骨科,江苏 南京 210011 
吕文莉 中国药科大学药学院,江苏 南京 210009 
侯冬枝 广东药学院药科学院,广东 广州 510006 
摘要点击次数: 1729
全文下载次数: 239
中文摘要:
      目的:研制肝靶向牛血清白蛋白(bovine serum albumin,BSA)脂质体,并研究其理化性质,为蛋白质药物的肝靶向给药提供一种新模型?方法:首先采用逆相蒸发法制备BSA负电荷脂质体,再用O-羧甲基-N-乳糖酰化壳聚糖(O-carboxymethyl N-galactosylated chitosan,Gal-CMCS)修饰,制备肝靶向BSA脂质体,并考察脂质体的形态?粒径?Zeta 电位?包封率及稳定性等理化性质?结果:制得的肝靶向BSA脂质体在电镜下呈类圆形,粒径分布均匀,平均粒径为(284.1 ± 5.41)nm,BSA包封率为(34.21 ± 1.73)%,Zeta 电位为(+27.1 ± 1.17)mV,4℃下放置3个月粒径和包封率无明显变化?结论:成功制备了肝靶向BSA脂质体,其具有良好的稳定性和较高的药物包封率,可作为蛋白质药物肝靶向给药的一种新模型?
英文摘要:
      Objective:To prepare the hepatic-targeted Bovine serum albumin(BSA)liposome and study its physicochemical properties, to providing a new hepatic-targeted drug delivery system for protein-drug. Methods:Liposome was prepared by reverse phase evaporation method,and then the liposome was coated with O-carboxymethyl N-galactosylated chitosan(Gal-CMCS). The shape,particle size,Zeta potential,entrapment efficiency and stability were investigated. Results:Hepatic-targeted BSA liposome was round and uniform;its mean particle size was(284.1±5.41)nm;the entrapment efficiency was(34.21±1.73)%; Zeta potential was(+27.1±1.17) mV;and there was no obvious change in entrapment efficiency and particle size at 4℃ within 3 months. Conclusion:The hepatic-targeted BSA liposome was successfully prepared,with good stability and high entrapment efficiency,thus providing a new hepatic-targeted drug delivery system for protein-drug.
查看全文   查看/发表评论  下载PDF阅读器
关闭