文章摘要
孟庆玲,黄 曙,季国忠,范志宁.Klotho基因G-395A和C1818T多态性与胃癌遗传易感性的研究[J].南京医科大学学报,2008,28(10):1258~
Klotho基因G-395A和C1818T多态性与胃癌遗传易感性的研究
The associations between the Klotho gene G-395A and C1818T polymorphisms and the risk of gastric cancer
投稿时间:2008-05-14  
DOI:10.7655
中文关键词: Klotho基因  胃癌  单核苷酸多态  易感性
英文关键词: Klotho gene  gastric cancer  single nucleotide polymorphisms  cancer risk
基金项目:南京医科大学自然科学基金重点项目(07NMUZ015)
作者单位
孟庆玲 南京医科大学第二附属医院消化医学中心,江苏 南京 210011 
黄 曙  
季国忠  
范志宁  
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中文摘要:
      目的:探讨Klotho基因单核苷酸多态性(SNPs)与江苏人群胃癌遗传易感性的关系?方法:采用病例-对照的分子流行病学方法,以聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法对151例胃癌和145例健康人群的Klotho基因启动子区域G-395A和外显子4区C1818T进行基因分型,并比较不同基因型与胃癌发病危险的关系?结果:G-395A多态性位点中GA+AA基因型在胃癌中的频率(39.7%)显著高于对照组(28.2%),携带GA+AA变异基因型者患胃癌的危险性是GG的1.66倍(调整的比值比:95℅可信区间:1.02~2.72)?分层分析显示,此危险在50岁以下的男性人群中更为显著,同时在吸烟和饮酒人群中也有显著的关系?C1818T多态性在胃癌和对照人群的分布无显著差别?两个多态性位点处于连锁不平衡(D = 0.449,r2 = 0.187),在对照人群中均符合Hardy-Weniberg平衡,4种单倍体在病例和对照组中的分布均无显著性差异? 结论:在中国江苏汉族人群中,Klotho基因启动子区多态位点G-395A变异显著增加江苏人群患胃癌的危险性,外显子4区C1818T多态性则无明显影响?
英文摘要:
      Objective:To investigate the association between Klotho gene polymorphisms and the risk of gastric cancer in Jiangsu population. Methods:In a case-control study of 151 patients and 145 controls,we genotyped G-395A in the promoter region and C1818T in exon 4 of Klotho gene by PCR-restriction fragment length polymorphism(PCR-RFLP) methods. The association between the genetic polymorphisms and the risk of the cancer was further examined by multivariate analysis. Results:The frequency of the A allele of G-395A polymorphism in patients(39.7%) was significantly higher than that in controls(28.2%,P = 0.038), with the adjusted OR=1.66 and 95℅CI 1.02~2.72,which was more pronounced among subgroups of youger males,smokers and drinkers. However,the frequency of C1818T did not show any significant difference between the patients and control subjects(P = 0.80). The two polymorphisms were in linkage disequilibrium(D = 0.449,r = 0.187) and in compliance with Hardy-Weniberg equilibrium in control. None of the haplotypes were significantly associated with the presence of the gastric cancer. Conclusion:The G-395A polymorphism in Klotho gene may positively contribute to the etiology of gastric cancer in Jiangsu population.
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