文章摘要
朱慕云,黄 茂.草分枝杆菌对SPCA/Ⅰ人肺腺癌细胞株诱导凋亡作用的研究[J].南京医科大学学报,2008,28(10):1272~
草分枝杆菌对SPCA/Ⅰ人肺腺癌细胞株诱导凋亡作用的研究
Study the effects of Mycobacterium Phlei on inducing apoptosis in SPCA/Ⅰ human lung adenocarcinoma cancer cells
  
DOI:10.7655
中文关键词: 草分枝杆菌  肺腺癌  凋亡
英文关键词: Mycobacterium Phlei  lung adenocarcinoma  apoptosis
基金项目:
作者单位
朱慕云 南京医科大学第一附属医院呼吸科,江苏 南京 210029 
黄 茂  
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中文摘要:
      目的:探讨草分子杆菌(Mycobacterium Phlei) 对SPCA/Ⅰ人肺腺癌细胞株的抗增殖效应及诱导细胞凋亡的作用?方法:将SPCA/Ⅰ人肺腺癌细胞株培养传代,分别加入不同浓度的草分子杆菌,作用36 h?分别以酶标仪检测细胞抑制率,FACSAria流式细胞仪检测细胞凋亡率,倒置显微镜观察凋亡细胞形态?结果:不同浓度的草分子杆菌体外对人肺腺癌细胞株SPCA/Ⅰ均有直接的抑制作用,其抑制率与所用药物浓度呈正相关?草分子杆菌体外对人肺腺癌细胞株SPCA/Ⅰ有诱导凋亡的作用,细胞凋亡率明显增加?倒置显微镜下可见细胞由原来贴壁生长而逐渐脱落?外形变圆?体积缩小?折光性增强,核染色质凝集,细胞裂解?结论:草分子杆菌能通过诱导细胞凋亡,直接抑制人肺腺癌细胞株SPCA/Ⅰ增殖?
英文摘要:
      Objective:To study the effects of Mycobacterium Phlei on cell apoptosis in SPCA/Ⅰ human lung cancer cells. Methods:The Cells were cultured in RPMI 1640 together with Mycobacterium Phlei at different concentrations for 36 hour. The changes of apoptosis of SPCA/Ⅰ human lung adenocarcinoma cells induced by Mycobacterium Phlei were detected by Annexin V: FITC Apoptosis detection kit,FACSAria flow cytometry and the inverted microscope. Results:The growth inhibitory rate had the positive correlations with concentrations of Mycobacterium Phlei on SPCA/Ⅰ human lung adenocarcinoma cells. Mycobacterium Phlei had significant effects on cell apoptosis in SPCA/Ⅰ human lung cancer cells. Typical apoptotic cells were seen whose bodies became smaller and rounder. Conclusion:Mycobacterium Phlei can induce apoptosis on SPCA/Ⅰ human lung adenocarcinoma cells and inhibit the tumor cell proliferation.
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