Objective:To establish the vascular blocking model in the left lung of rabbit and investigate the lung ischemia-reperfusion injury in pulmonary surgery. Methods:96 New Zealand rabbits were randomly divided into 3 groups:groupⅠ, control group; groupⅡ, blocking pulmonary artery; groupⅢ, blocking pulmonary artery and vein. After the time of opening chest(groupⅠ),1hour’s ischemia and 1,2,4,6 hours, reperfusion ,the changes of partial pressure of oxygen in arterial blood(PaO2) and the content of MDA in left lung tissue were observed. Then the water content of left lung and pathological study were recorded. Results:Homodynamic parameters were stable in all 3 groups. There were significant differences in PaO2, MDA and wet/dry ratio of the lung tissue between groupⅠand the other two groups(P < 0.05 and P < 0.01),and there was no significant difference between group Ⅱ and group Ⅲ(P > 0.05). Pathological study revealed that similar injury changes happened between group Ⅱ and groupⅢ, and the obvious injury happened at the time of 4 hours, reperfusion. At the time of 6 hours, reperfusion pathological changes in both group Ⅱ and group Ⅲ began to recover. Conclusion:This provides a simple, reliable and inexpensive model for investigating the lung ischemia-reperfusion injury in pulmonary surgery.