文章摘要
陈 建,许 林,蒋 峰,丁忠海,杨 民,张 彤.肺外科中兔肺缺血-再灌注损伤模型的建立[J].南京医科大学学报,2008,28(10):1312~1315
肺外科中兔肺缺血-再灌注损伤模型的建立
Establishment of rabbit lung ischemia-reperfusion injury model in pulmonary surgery
投稿时间:2008-04-22  
DOI:10.7655
中文关键词: 肺外科  缺血-再灌注损伤  动物模型  
英文关键词: pulmonary surgery  ischemia-reperfusion injury  animal model  rabbit
基金项目:
作者单位
陈 建 江苏省肿瘤医院胸外科,江苏 南京 210009 
许 林 江苏省肿瘤医院胸外科,江苏 南京 210009 
蒋 峰 江苏省肿瘤医院胸外科,江苏 南京 210009 
丁忠海 江苏省肿瘤医院胸外科,江苏 南京 210009 
杨 民 江苏省肿瘤医院胸外科,江苏 南京 210009 
张 彤 江苏省肿瘤医院病理科,江苏 南京 210009 
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中文摘要:
      目的:建立兔单侧肺血管阻断后再开放模型,用于探讨肺外科中肺缺血-再灌注损伤的特点?方法:同种新西兰白兔96只,随机分成3组,Ⅰ组为对照组(n = 36);Ⅱ组为单纯阻断肺动脉组(n = 30);Ⅲ组为阻断肺循环组(n = 30)?术中监测动物血流动力学指标;分别于开胸时(仅Ⅰ组)?缺血1 h和再灌注1?2?4?6 h测定左肺静脉血氧分压;检测肺组织中丙二醛(MDA)含量;取肺组织作病理学观察,并测定手术侧肺湿/干比?结果:各组动物术中血流动力学指标平稳;在缺血1 h和再灌注1?2?4?6 h,Ⅰ组与Ⅱ?Ⅲ组相比,氧分压?肺湿/干重比及肺组织中MDA含量的差异均有显著性(P < 0.05和P < 0.01),Ⅲ组与Ⅱ组相比,差异无显著性(P > 0.05);Ⅱ?Ⅲ组呈现相似的肺损伤病理改变过程,均在再灌注4 h出现较为明显的肺损伤,再灌注6 h肺损伤呈恢复趋势?结论:该方法的建立为进一步研究肺外科中肺缺血-再灌注损伤提供了较为理想的动物模型?
英文摘要:
      Objective:To establish the vascular blocking model in the left lung of rabbit and investigate the lung ischemia-reperfusion injury in pulmonary surgery. Methods:96 New Zealand rabbits were randomly divided into 3 groups:groupⅠ, control group; groupⅡ, blocking pulmonary artery; groupⅢ, blocking pulmonary artery and vein. After the time of opening chest(groupⅠ),1hour’s ischemia and 1,2,4,6 hours, reperfusion ,the changes of partial pressure of oxygen in arterial blood(PaO2) and the content of MDA in left lung tissue were observed. Then the water content of left lung and pathological study were recorded. Results:Homodynamic parameters were stable in all 3 groups. There were significant differences in PaO2, MDA and wet/dry ratio of the lung tissue between groupⅠand the other two groups(P < 0.05 and P < 0.01),and there was no significant difference between group Ⅱ and group Ⅲ(P > 0.05). Pathological study revealed that similar injury changes happened between group Ⅱ and groupⅢ, and the obvious injury happened at the time of 4 hours, reperfusion. At the time of 6 hours, reperfusion pathological changes in both group Ⅱ and group Ⅲ began to recover. Conclusion:This provides a simple, reliable and inexpensive model for investigating the lung ischemia-reperfusion injury in pulmonary surgery.
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