文章摘要
方 奕,邢昌赢,王 涛,周富华.水飞蓟宾对转化生长因子β1诱导的人肾小管上皮细胞中结缔组织生长因子及Ⅲ型胶原表达的影响[J].南京医科大学学报,2008,28(11):1381~1384
水飞蓟宾对转化生长因子β1诱导的人肾小管上皮细胞中结缔组织生长因子及Ⅲ型胶原表达的影响
The effect of silibinin on the expression of connective tissue growth factor(CTGF) and the type Ⅲ collagen in HK-2 cells induced by TGF-β1
投稿时间:2008-06-20  
DOI:10.7655
中文关键词: 水飞蓟宾  转化生长因子-β1  结缔组织生长因子  Ⅲ型胶原
英文关键词: silibinin  TGF-β1  connective tissue growth factor  type Ⅲ collagen
基金项目:南京医科大学科技发展基金面上项目(06NMUM030)
作者单位
方 奕 南京医科大学第一附属医院肾内科,江苏 南京 210029 
邢昌赢  
王 涛  
周富华  
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中文摘要:
      目的:了解水飞蓟宾(silibinin)对转化生长因子(TGF)-β1诱导的人近曲肾小管上皮细胞(HK-2)结缔组织生长因子(CTGF)表达及Ⅲ型胶原分泌的影响?方法:将体外培养的HK-2细胞分为3组:①对照组;②TGF-β1组(TGF-β1的终浓度为5 ng/ml);③TGF-β1+水飞蓟宾组(TGF-β1的终浓度为5 ng/ml,水飞蓟宾终浓度分别为5?10?20 μg/ml )?48 h后,采用逆转录聚合酶链反应(RT-PCR)检测细胞内CTGFmRNA的水平,ELISA方法检测培养上清中胶原Ⅲ及CTGF的浓度?结果:与对照组比较,TGF-β1刺激使HK-2 细胞CTGF基因表达和蛋白分泌及Ⅲ型胶原分泌均显著增加(P < 0.01)?与TGF-β1组比较,加用5~20 μg/ml水飞蓟宾可以抑制TGF-β1刺激所诱导的CTGF基因表达和蛋白分泌及Ⅲ型胶原分泌(P < O.01),以20 μg/ml 水飞蓟宾效果最显著?结论:水飞蓟宾能部分抑制TGF-β1诱导CTGF的基因和蛋白的表达及Ⅲ型胶原分泌,提示水飞蓟宾可能通过阻抑CTGF表达及减少细胞外基质的积聚,具有潜在的抗肾纤维化作用?
英文摘要:
      Objective:To investigate the effect of silibinin on the expression of connective tissue growth factor(CTGF) and the secretion of type Ⅲ collagen in HK-2 cells induced by TGF-β1. Methods:In vitro,HK-2 cells were divided into three groups:①control group;②5 ng/ml TGF-β1 group; ③5 ng/ml TGF-β1+silibinin with different concentrations group, 5 μg/ml,10 μg/ml and 20 μg/ml,respectively. 48 h later,the expression of CTGF mRNA in cells were detected by reverse transcription-po1ymerase chain reaction(RT-PCR);the concentrations of CTGF and collagen Ⅲ secreted into the culture supernatant was determined by ELISA. Results:Compared with control group,the expression of CTGF mRNA and the concentration of the secreted collagen Ⅲ and CTGF were all increased markedly in the group of TGF-β1 treatment(P < 0.01). Compared with TGF-β1 group,the different concentrations of(5 μg/ml,10 μg/ml and 20 μg/ml) silibinin could also effectively suppress the expressions of CTGF mRNA and the concentration of the secreted collagen Ⅲ and CTGF(P < 0.01) in a dose-dependent manner. And the 20 μg/ml silibinin group had the most prominent effect. Conclusion:Silibinin could suppress the expressions of collagen Ⅲ and CTGF induced by TGF-β1. It is suggested that silibinin might exert an inhibitive effect on renal fibrosis,which may be related with its role in reducing the expression of CTGF and preventing accumulation of ECM.
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