文章摘要
解 鹏,李 玥,陈 琪.表面等离子共振法测?茁-淀粉样多肽人源性单链抗体E3 scfv的亲和力[J].南京医科大学学报,2008,28(11):1389~1392
表面等离子共振法测?茁-淀粉样多肽人源性单链抗体E3 scfv的亲和力
Determination of affinity of human single-chain variable fragment antibody against ?茁-amyloid peptide by SPR
投稿时间:2008-07-20  
DOI:10.7655
中文关键词: ?茁-淀粉样多肽  单链抗体  表面等离子共振  亲和力
英文关键词: ?茁-amyloid peptide  single-chain antibody  surface plasmon resonance  affinity
基金项目:江苏省教育厅产业化项目(JH01-048)
作者单位
解 鹏 南京医科大学动脉粥样硬化研究中心,江苏 南京 210029 
李 玥  
陈 琪  
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中文摘要:
      目的:采用表面等离子共振(surface plasmon resonance,SPR)生物传感器测定?茁-淀粉样多肽(A?茁)人源性单链抗体E3 scfv的亲和力?方法:将A?茁1-40固定于传感芯片表面,以E3 scfv等抗体为流动相,实时监测抗原抗体的结合和解离过程,并对其相互作用模式和动力学常数进行分析?结果:E3 scfv?阳性对照抗体DE2B4均能与A?茁1-40发生特异性免疫结合反应,阴性对照抗体9E10则不能结合A?茁1-40;A?茁1-40与E3 scfv及DE2B4的结合呈现快结合慢解离的动力学过程,其中DE2B4与A?茁1-40的亲和力为6.77 × 10-7 mol/L,E3 scfv的亲和力为5.38 × 10-6mol/L?结论:SPR适宜用作测定E3 scfv与A?茁1-40的亲和力?
英文摘要:
      Objective:To investigate the affinity of human single-chain variable fragment antibody against ?茁-amyloid peptide(A?茁) by surface plasmon resonance(SPR) sensor. Methods:A?茁1-40 was fixed on sensing chip surface and the antibodies as E3 scfv were applied as mobile phase. Binding and separation of A?茁1-40 with the antibodies were monitored in real time. The interaction mode between antigen and antibody and the relevant dynamic parameters were determined. Results:A specific interaction between A?茁1-40 and E3 scfv or DE2B4,rather than 9E10 was detected. The binding and separation between the antigen and antibody were dynamic with a slow speed; Affinity DE2B4 with A?茁1-40 was 6.77 × 10-7 mol/L and that of E3 scfv was 5.38 × 10-6 mol/L. Conclusion:SPR is useful in determination of affinity of E3 scfv with A?茁1-40.
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