文章摘要
肖 岳,郑 帼,卢孝鹏,张爱华,吴春风.海人酸癫疒大鼠海马存在能量代谢异常和线粒体功能障碍[J].南京医科大学学报,2008,28(11):1468~1470
海人酸癫疒大鼠海马存在能量代谢异常和线粒体功能障碍
Mitochondrial dysfunction and changes of energy metabolism in kainite acid-induced epilepsy in rat
投稿时间:2008-05-29  
DOI:10.7655
中文关键词: 海人酸  海马  线粒体功能障碍
英文关键词: kainite acid  hippocampus  mitochondrial dysfunction
基金项目:南京市科技发展重大项目(ZKX0408)
作者单位
肖 岳 南京医科大学附属南京儿童医院急诊科,江苏 南京 210008 
郑 帼 南京医科大学附属南京儿童医院神经科,江苏 南京 210008 
卢孝鹏 南京医科大学附属南京儿童医院肾科,江苏 南京 210008 
张爱华 南京医科大学附属南京儿童医院肾科,江苏 南京 210008 
吴春风 南京医科大学附属南京儿童医院神经科,江苏 南京 210008 
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中文摘要:
      目的:观察海人酸(KA)颞叶癫■大鼠海马细胞内游离钙离子浓度?线粒体膜电位及线粒体酶活性和细胞能量代谢的改变,探讨线粒体功能障碍在海马神经元凋亡中的作用?方法:海人酸颞叶癫■大鼠模型采用一侧海马注射海人酸制备?40只SD大鼠随机分为生理盐水对照组和KA组(6 h?1?3?7天),每组8只?检测各组海马细胞内游离钙离子浓度?线粒体膜电位?线粒体呼吸链复合物Ⅱ和Ⅳ以及Na+-K+-ATP酶活性?三磷酸腺苷(ATP)和二磷酸腺苷(ADP)的浓度?结果:KA组大鼠海马细胞内游离钙离子浓度显著增加,线粒体膜电位?线粒体呼吸链复合物Ⅱ和Ⅳ以及Na+-K+-ATP酶活性?ATP浓度?ATP/ADP比值显著降低,且随时间延长,其下降尤为显著?结论:KA大鼠海马存在能量代谢异常和线粒体功能障碍?
英文摘要:
      Objective:To study the effect of mitochondrial dysfunction on hippocampal cell apoptosis in kainite acid-induced epilepsy in rat. Methods:Kainite acid-induced epilepsy model was induced by injection of kainite acid into the hippocampus. Forty SD rats were randomly divided into five groups: the control and KA group(6 h,1 d,3 d,7 d),n = 8 for each group. The concentration of free calcium ion,ATP,and ADP,mitochondrial membrane potential,the activities of the mitochondrial respiratory chains complexⅡand Ⅳ,Na+-K+-ATPase in hippocampus were detected. Results:In the hippocampus,the concentration of free calcium ion was increased significantly,and mitochondrial membrane potential,the activities of the mitochondrial respiratory chains complexⅡand Ⅳ,Na+-K+-ATPase,and the concentration of ATP were significantly decreased in a time-dependent manner. Conclusion:Mitochondrial dysfunction may cause hippocampal cell apoptosis in kainite acid-induced epilepsy in rat.
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