文章摘要
苏建华,李慧敏,缪 珩,鲁一兵,林 志,蒋仁俐.罗格列酮对高糖状态下人脐静脉内皮细胞血红素加氧酶-1表达的影响[J].南京医科大学学报,2008,28(12):1551~1554
罗格列酮对高糖状态下人脐静脉内皮细胞血红素加氧酶-1表达的影响
Effect of rosiglitazone on the expression of HO-1 and cell survival of HUVECs cultured in high glucose condition
投稿时间:2008-07-03  
DOI:10.7655
中文关键词: 罗格列酮  人脐静脉内皮细胞  高糖  血红素加氧酶-1
英文关键词: rosiglitazone  HUVECs  high glucose  heme oxygenase-1
基金项目:江苏省卫生厅科技发展重点课题(H200514)
作者单位
苏建华 南京医科大学附属无锡二院老年医学科,江苏 无锡 214002 
李慧敏 南京医科大学附属无锡二院老年医学科,江苏 无锡 214002 
缪 珩 南京医科大学附属无锡二院老年医学科,江苏 无锡 214002 
鲁一兵 南京医科大学附属无锡二院老年医学科,江苏 无锡 214002 
林 志 南京医科大学第二附属医院内分泌科,江苏 南京 210011 
蒋仁俐 南京医科大学附属无锡二院老年医学科,江苏 无锡 214002 
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中文摘要:
      目的:观察罗格列酮对高糖状态下人脐静脉内皮细胞(HUVECs)血红素加氧酶-1(HO-1)表达及细胞活力的影响?方法:体外培养HUVECs,分别在不同时间和用不同浓度的罗格列酮干预高糖状态下培养的HUVECs,提取细胞总RNA和蛋白,RT-PCR和Western blot 检测HO-1表达的改变?采用台盼蓝染色法,检测细胞死亡率的变化?结果:与0 μmol/L罗格列酮组及作用0 h组相比,罗格列酮呈时间和浓度依赖性,提高高糖状态下HUVECs的HO-1表达,差别均具有统计学意义(P < 0.05)?罗格列酮作用组HUVECs死亡率明显下降,差别均具有统计学意义(P < 0.05)?结论:罗格列酮可以提高内皮细胞在高糖状态下抗氧化应激的能力,起到保护血管的作用?
英文摘要:
      Objective:To investigate the effect of rosiglitazone on the expression of heme oxygenase-1 and cell survival in human umbilical vein endothelial cells cultured in high glucose condition. Methods:HUVECs in vitro were treated with rosiglitazone at different concentrations(ranging from 0 to 30 μmol/L) for 48 h or exposed to10 μmol/L rosiglitazone for 0?24,48 and 72 h,respectively. Gene expression was analyzed at mRNA level by RT-PCR or at protein level by Western blot. The trypan-blue exclusion test was used to study the influence of rosiglitazone on cell survial. Results:Compared to the control groups,the expression of HO-1 was induced by rosiglitazone in a dose and time-dependent manner(P < 0.05),and it could make the cell survival increase(P < 0.05). Conclusion:Rosiglitazone could induce the expression of HO-1 gene in HUVECs cultured in high glucose condition and make cell death atc of endothelial cells decrease. This indicated that rosiglitazone could elevate the capability of the cells counteracting the oxidative stress in high glucose condition,which could protect the vascular function.
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