文章摘要
巩玉森,仇 玮,李琳琳,柳 红,吴永平.As2O3与阿司匹林联合应用增强对人胃癌SGC-7901细胞增殖的抑制作用[J].南京医科大学学报,2008,28(12):1555~1559
As2O3与阿司匹林联合应用增强对人胃癌SGC-7901细胞增殖的抑制作用
Inhibitory effects of As2O3 and Aspirin on cell proliferation in human gastric carcinoma cell line SGC-7901
投稿时间:2008-08-15  
DOI:10.7655
中文关键词: As2O3  阿司匹林  SGC-7901细胞
英文关键词: As2O3  Aspirin  SGC-7901 cell
基金项目:江苏省教育厅科研课题(06KJB310118)
作者单位
巩玉森 徐州医学院病理学教研室,江苏 徐州 221002 
仇 玮  
李琳琳  
柳 红  
吴永平  
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中文摘要:
      目的:探讨As2O3与阿司匹林联合应用对人胃癌SGC-7901细胞生长的影响?方法:采用MTT法检测As2O3和阿司匹林对SGC-7901细胞株增殖的影响,采用流式细胞术检测两者对SGC-7901细胞凋亡和细胞周期分布的影响?结果:①药物作用后72 h,4 μmol/L和 2 mmol/L As2O3组生长抑制率分别为31.04%及16.11%,与阴性对照组相比差异具有统计学意义(P < 0.05);2 μmol/L As2O3+ 1 mmol/L阿司匹林联合用药组的生长抑制率为24.19%,与阴性对照组? 2 μmol/L As2O3(5.96%)及1 mmol/L阿司匹林(4.86%)相比,差异均有统计学意义(P < 0.05);②2 μmol/L As2O3与1 mmol/L阿司匹林联合组细胞的凋亡率是19.01%,与阴性对照组(1.55%)?2 μmol/L As2O3组(1.81%)及1 mmol/L阿司匹林组(1.64%)相比,差异均有统计学意义(P < 0.05);③As2O3使细胞G0/G1期比例下降,G2/M期比例增加?阿司匹林使细胞G0/G1期比例增加,S和G2/M期比例下降?2 μmol/L As2O3与1 mmol/L阿司匹林联合应用后72 h,G0/G1期和G2/M期比例增加,S期比例下降?结论:As2O3?阿司匹林可以抑制SGC-7901细胞增殖?诱导其凋亡,阻滞细胞周期,两者联合应用具有协同作用,可以同时降低两药的用量?
英文摘要:
      Objective:To investigate the inhibitory effects of As2O3 and Aspirin on cell proliferation in human gastric carcinoma cells(SGC-7901 cell lines). Methods:The inhibition of proliferation,apoptosis and cell cycle of gastric cancer cells SGC-7901 were detected by MTT assay and the flow cytometry and light microscope. Results:①After 72 hours,MTT assay showed the growth of the SGC-7901 cells was inhibited by As2O3(4 μmol/L), Aspirin(2 mmol/L),and As2O3+Aspirin(2 μmol/L + 1 mmol/L). And the inhibitory rates were 31.04%,16.11% and 24.19% respectively. It also showed the inhibitory effects among negative control group,two single drug groups(As2O3 group,Aspirin group) and drug combination group(P < 0.05). ②Flow cytometry showed the apoptosis rates were 1.55% in negative group,1.81% in As2O3 group,1.64% in Aspirin group and 19.01% in As2O3+Aspirin group,which showed statistical significance(P < 0.05). ③The proportion of cells at G0/G1 phase was significantly decreased while the proportion of cells at G2/M phase was increased in As2O3 group. The proportion of cells at G0/G1 phase was significantly increased while the proportion of cells at S phase and G2/M phase were decreased in Aspirin group. And the increasing of proportion of cells at G0/G1 phase and G2/M phase were found in drug combination group,while proportion of cells at S phase decreased. Conclusion:As2O3 and Aspirin could inhibit the growth of gastric cancer cell SGC-7901 by inducing apoptosis and cell cycle arrest. It showed that inhibitory effect on proliferation of SGC-7901 cell line in drug combination group(As2O3+Aspirin) were markedly enhanced and their dose was decreased.
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