文章摘要
谷 翔,黄问银,冯义柏.胰岛素对大鼠缺血后再灌注心肌细胞凋亡?Bad表达影响及机制研究[J].南京医科大学学报,2008,28(12):1560~
胰岛素对大鼠缺血后再灌注心肌细胞凋亡?Bad表达影响及机制研究
Role of Bad in anti-apoptotic mechanism of insulin during post-ischemia reperfusion myocardium injury in rats
投稿时间:2008-05-09  
DOI:10.7655
中文关键词: 胰岛素  细胞凋亡  Bad  信号转导
英文关键词: insulin  apoptosis  Bad  signal pathway
基金项目:
作者单位
谷 翔 九江学院附属医院心内科,江西 九江 332000 
黄问银 九江学院附属医院心内科,江西 九江 332000 
冯义柏 华中科技大学同济医学院附属协和医院心内科,湖北 武汉 430022 
摘要点击次数: 1455
全文下载次数: 185
中文摘要:
      目的:观察胰岛素对大鼠缺血后再灌注心肌细胞凋亡及促凋亡蛋白Bad磷酸化表达的影响,以进一步探讨胰岛素在缺血再灌注损伤中的抗凋亡机制?方法:46只SD雄性大鼠随机分为四组:缺血再灌注对照组(I/R,n = 12),实施30 min缺血/180 min再灌注;胰岛素组(INS,n = 12),于再灌注期前5 min颈静脉内输注胰岛素(60 U/L,每小时4 ml/kg)至灌注期结束;胰岛素+LY294002组(INS+LY,n = 12),再灌注前10 min静脉注射LY294002(0.3 mg/kg),5 min后再静脉输注胰岛素+LY294002(每小时30 ?滋g/kg);假手术组(SHAM,n = 10):仅给予冠脉穿线,不实施结扎?实验结束后,用原位末端标记法(TUNEL)和DNA梯带法(DNA Ladder)标测细胞凋亡,用免疫组化和Western-blotting法检测磷酸化Bad(pBad)表达?结果:①与SHAM组相比,I/R组凋亡指数(AI)明显增加(P < 0.01),pBad表达降低(P < 0.05);②与I/R组相比,INS组AI值明显减少(P < 0.01),pBad表达升高(P < 0.01);③与INS组相比,INS+LY组AI值明显增加(P < 0.01),pBad表达降低(P < 0.05)?结论:①缺血再灌注可导致心肌组织pBad低表达;②胰岛素对大鼠在体缺血再灌注心肌有明显抗凋亡作用;③PI3K/Akt/Bad途径可能为胰岛素抗缺血再灌注诱导心肌凋亡的重要信号转导机制,其机制可能与胰岛素通过PI3K/Akt途径减少pBad表达有关?
英文摘要:
      Objective:To observe anti-apoptotic effect of insulin,and explore the role of Bad in anti-apoptotic mechanism of insulin during post-ischemia reperfusion injury in myocardium of rats. Methods:Forty-six male Sprague-Dawley rats were randomly divided into 4 groups:①I/R group was subjected to ischemia for 30 min and reperfusion for 180 min;②INS group was subjected to intravenous infusion of Insulin(60 U/L,4 ml/kg·h) at 5 min before 3 h of reperfusion;③INS+LY group was subjected to intravenous infusion of LY294002(0.3 mg/kg) at 10 min before 3 h of reperfusion and intravenous infusion of insulin and LY294002(30 ?滋g·kg-1·h-1) at 5 min before 3 h of reperfusion;④SHAM group was subjected to placing a silk around the left anterior descending coronary artery without a slipknot. At the end of 4 h of reperfusion,apoptosis of cardiomyocytes were assessed through TUNEL and DNA Ladder;Immunochemistry and Western-blotting were used to analyse the expression of phosphorylated Bad in all groups. Results:①Compared with I/R group,apoptosis index(AI) were significantly decreased,and expression of phosphorylated Bad was prominently higher in INS group. ②There were a remarkably increase in AI and lower expression of phosphorylated Bad in INS+LY group than that in INS group. Conclusion:①Ischemia and reperfusion resulted in lower expression of phosphorylated Bad in myocardium of rats;②Insulin exerted an anti-apoptotic effect in myocardium of rats during ischemia and reperfusion;③its mechanism may be involved in higher expression of phosphorylated Bad via PI3-K/Akt pathway.
查看全文   查看/发表评论  下载PDF阅读器