文章摘要
闻晓烽,沈美萍,刘翠萍,矛晓东.曲古抑菌素A对甲状腺未分化癌DRO细胞株生长及其端粒酶逆转录酶mRNA表达影响的研究[J].南京医科大学学报,2008,28(12):1565~1568
曲古抑菌素A对甲状腺未分化癌DRO细胞株生长及其端粒酶逆转录酶mRNA表达影响的研究
Effects of Trichostatin A on hTERT-mRNA expression and proliferation in human anaplastic thyroid carcinoma cell
投稿时间:2008-06-10  
DOI:10.7655
中文关键词: 曲古抑菌素A  甲状腺未分化癌  细胞增殖  端粒酶催化亚单位
英文关键词: Trichostatin A  anaplastic thyroid carcinoma  human telomerase reverse transcriptase
基金项目:
作者单位
闻晓烽 南京医科大学第一附属医院普外科,江苏 南京 210029 
沈美萍  
刘翠萍  
矛晓东  
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中文摘要:
      目的:观察曲古抑菌素A(TSA)对甲状腺未分化癌DRO细胞株的体外作用,探讨TSA对甲状腺未分化癌的作用机制?方法:倒置相差显微镜观察细胞形态变化;MTT法检测TSA对DRO细胞增殖的抑制效应;流式细胞术检测经TSA1.0 ?滋mol/L作用24?48?72 h后细胞周期和凋亡率的变化;RT-PCR方法观察经TSA 1.0 ?滋mol/L作用48 h后细胞内hTERT和p21 基因的表达情况?结果:在0.1~1.5 ?滋mol/L TSA作用下,DRO细胞生长明显受抑制;倒置相差显微镜检测DRO细胞经TSA处理后形态发生明显变化;1.0 ?滋mol/L TSA作用于DRO细胞,48 h后出现明显的细胞周期阻滞;hTERT经TSA诱导后表达明显下调,呈量效关系?结论:一定浓度的TSA可以抑制甲状腺未分化癌细胞株DRO增殖?其作用机制可能与下调hTERT表达有关?
英文摘要:
      Objective:To investigate the effect of Trichostatin A on the growth of human anaplastic thyroid carcinoma cell line in vitro. Methods:Human anaplastic thyroid carcinoma DRO cell line was treated with Trichostatin A of different concentrations and time. The antiproliferative effect was measured by methyl thiazolyl tetrazolium(MTT). Flow cytometry was used to examine apoptosis and cell cycle when TSA was 1.0 ?滋mol/L in concentration and the exposure to DRO was 24 h,48 h and 72 h,respectively. The leves of hTERT-mRNA,p21-mRNA in cells were detected by RT-PCR,with TSA for 48 h. Results:After being treated with 0.1 ?滋mol/L,0.2 ?滋mol/L,0.5 ?滋mol/L,1.0 ?滋mol/L,and 1.5 ?滋mol/L TSA,the prolifergation of DRO cells was significantly inhibited. IPCM showed that there were obvious morphologic changes after TSA treatment. Changes in the cells cycle occurred 48 h after treatment. hTERT expression was down-regulated after treatment with TSA. Conclusion:TSA significantly inhibited growth of the DRO cells and mechanism may be related to a down-regulation in hTERT expression.
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