文章摘要
亢 健,夏建国,姜 红,李俊芳.B7-1基因转染抑制胃癌生长和转移的实验研究[J].南京医科大学学报,2009,29(1):7~11
B7-1基因转染抑制胃癌生长和转移的实验研究
Inhibition of growth and metastasis of gastric cancer by transfection of B7-1
投稿时间:2008-08-01  
DOI:10.7655
中文关键词: B7-1  SGC-7901  淋巴转移  免疫基因治疗
英文关键词: B7-1  SGC-7901  lymph node metastasis  immunogene therapy
基金项目:江苏省卫生厅社会发展基金资助项目(J200624)
作者单位
亢 健 南京医科大学第一附属医院胃肠外科,江苏 南京 210029 
夏建国 南京医科大学第一附属医院胃肠外科,江苏 南京 210029 
姜 红 南京医科大学第一附属医院胃肠外科,江苏 南京 210029 
李俊芳 镇江卫生学校,江苏 镇江 212004 
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中文摘要:
      目的:研究B7-1基因转染胃癌细胞对人胃癌生长和转移的影响?方法:将重组质粒B7-1pcDNA3.1(+)和空载体pcDNA3.1(+)分别转染SGC-7901细胞(简称S),用G418筛选分别建立稳定转染细胞株SGC-7901/B7-1pcDNA3.1(+)(简称S-B)和SGC-7901/pcDNA3.1(+)(简称S-P)?RT-PCR和流式细胞术分别从mRNA和蛋白水平检测B7-1基因表达,检测细胞周期分布,计算细胞增殖指数?分离人外周血单个核细胞(PBMC),分别与S-B?S-P?S共培养,检测PBMC黏附力和杀伤力,检测胃癌细胞凋亡情况?将S-B?S-P?S分别接种于BALB/c裸鼠胃大弯侧,观察B7-1基因转染对胃癌生长及淋巴转移的影响?结果:S-B高表达B7-1基因,S-P和S不表达B7-1基因?S-B?S-P?S增殖指数无显著差异?S-B与PBMC黏附力和杀伤力均明显高于S-P和S,而S-P与S无显著差异?种植S-B小鼠成瘤率和转移率均低于S-P和S,HE染色见淋巴细胞广泛浸润于S-B种植形成肿瘤组织及转移淋巴结?结论:B7-1基因提高胃癌细胞免疫原性抑制胃癌生长和淋巴转移?
英文摘要:
      Objective:To investigate the influence on the growth and metastasis of gastric cancer by transfection of B7-1. Methods: Plasmids B7-1pcDNA3.1(+) and pcDNA3.1(+) were transfected into SGC-7901,then selected by G418 to establish the stable cell lines S-B and S-P. The expression of B7-1 mRNA and protein was detected, and then the distribution of cell cycle and the proliferation index(PI) was tested. Human peripheral blood mononuclear cells(PBMC) were separated and co-cultured with gastric cancer cell lines whose adhesion and cytotoxicity were tested Apoptosis condition of gastric cancer cells were also monitored. Gastric cancer cells were inoculated into the great curve of BALB/c nude mouse close to the gastric antrum Growth and metastasis of the gastric cancer were observed. Results:B7-1 was high expressed in S-B, but wasn’t expressed in S-P and S. S-B,S-P and S didn’t have significant difference in proliferation index. Adhesion and cytotoxicity of PBMC to S-B were stronger than that of S-P and S, but no significant difference between S-P and S. Tumorigenesis and lymph node metastasis of S-B were both lower than that of S-P and S. Lymphocytes were intensively infiltrated in both the S-B tumors and its metastatic lesions, however, were scarcely observed in the lesions associated with S-P and S. Conclusion:The transfection of B7-1 enhanced the immunogenicity of gastric cancer cell and inhibited its growth and lymph node metastasis.
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