抑抗汤调节不明原因不孕症患者子宫内膜白血病抑制因子表达的研究
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江苏省兴卫工程重点人才基金(RC2007070)


The effect of YiKang decoction on LIF expression of human endometrium of unexplained infertile women
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    目的:通过研究抑抗汤对不明原因不孕症患者子宫内膜白血病抑制因子(leukemia inhibitory factor,LIF)表达的影响,了解抑抗汤治疗不明原因不孕症的作用机制-方法:不明原因不孕症患者20例,口服标准量抑抗汤2个月,首先应用RT-PCR初步了解不孕症患者服药前-后黄体晚期子宫内膜表达LIFmRNA水平的差异,然后采用实时荧光定量PCR对服药前后LIF基因转录水平定量验证,并以免疫组化鉴定其在服药前后子宫内膜蛋白水平的表达情况-结果:不孕症患者治疗前-后子宫内膜LIF 荧光定量PCR的Ct值分别为9.79 ± 0.91和4.60 ± 1.24,两者相比差异有显著性(P < 0.01),表明治疗后LIF的转录水平高于治疗前-免疫组化结果显示LIF表达在子宫内膜腺上皮细胞的细胞浆内,且治疗后蛋白水平的表达较治疗前明显增强(P < 0.05)-结论:抑抗汤治疗不明原因不孕症可能是通过促进子宫内膜LIF的表达来实现的-

    Abstract:

    Objective:To study the effect of YiKang decoction on expression of leukemia inhibitory factor(LIF) in human endometrium of unexplained infertile women, and explore the possible mechanism of YiKang decoction for infertily treatment. Methods:Twenty infertile patients were treated with standard dose of YiKang decoction for two months. LIF mRNA levels in the endometrium of late luteal period were detected by RT-PCR and Real-time quantitative PCR. The protein levels and expression sites of LIF were subsequently confirmed by Immunohistochemistry(IHC). Results:The real-time quantitative PCR Ct value for LIF mRNA before and after treatment were 9.79 ± 0.91 and 4.60 ± 1.24, respectively, suggesting that the transcription level of LIF was higher after treatment(P < 0.01). IHC analysis also revealed that LIF was highly expressed at protein level(P < 0.05) and mainly seen in the cytoplasm of endometrium glandulose cells. Conclusion:These results indicated that YiKang decoction may cure the infertily through increasing expression of LIF in the endometrium.

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陈 健,应小燕,吴 难.抑抗汤调节不明原因不孕症患者子宫内膜白血病抑制因子表达的研究[J].南京医科大学学报(自然科学版),2009,29(2):195-199

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  • 收稿日期:2008-10-08
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