文章摘要
王亦秋,顾 民,龚永光,冯明明.睾丸去精索神经支配对大鼠性激素的影响[J].南京医科大学学报,2009,29(3):291~295
睾丸去精索神经支配对大鼠性激素的影响
Influence of testicular denervation on sexual hormone levels in adult rats:a time course study
投稿时间:2008-09-11  
DOI:10.7655
中文关键词: 睾丸  去神经支配  性激素
英文关键词: testis  denervation  sexual hormone
基金项目:国家自然科学基金资助(30400161)
作者单位
王亦秋 南京医科大学第一附属医院泌尿外科,江苏 南京 210029 
顾 民  
龚永光  
冯明明  
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中文摘要:
      目的:研究睾丸去神经支配对性激素水平的影响,探讨精索神经对精子发生的调控机制?方法:健康成年雄性SD大鼠48只,随机分为假手术组(SO)?精索上神经切除组(SSN)?精索下神经切除组(ISN)和精索上神经+精索下神经切除组(SSN+ISN),每组12只大鼠?解剖显微镜下建立睾丸去神经支配大鼠模型,分别于术前7天?术后1?7?14?21?28天于大鼠眼眶后静脉丛处取血,放射性免疫的方法检测血清中睾酮(T)?卵泡刺激素(FSH)?黄体生成素(LH)?泌乳素(PRL)和雌二醇(E2)水平?结果:术后1天各组血清T水平均显著下降(P < 0.05),术后7天回升,此后去神经组T水平持续下降(P < 0.05),SSN+ISN组较SSN组和ISN组下降更为显著(P < 0.05),SO组无明显变化(P > 0.05);术后1天各组血清LH水平均显著升高(P < 0.05),术后7天稍有回落,此后去神经组LH水平持续上升,SSN+ISN组较SSNG和ISN组上升更为显著,SO组无明显变化(P > 0.05);各组血清中FSH水平均于术后1天显著升高(P < 0.05),后逐渐恢复至术前水平;去神经组血清中PRL水平于手术后显著升高(P < 0.05),SO组无明显改变(P > 0.05);术后去神经组血清E2水平均显著下降(P < 0.05),SO组仅术后1天显著下降(P < 0.05),后恢复到术前水平(P > 0.05)?结论:精索神经对多种性激素水平具有调节作用,这可能是其实现生精调控作用的重要途径之一?
英文摘要:
      Objective:To further explore the regulatory role of the spermatic nerves in spermatogenesis by investigating effects of testicular denervation on sexual hormone levels. Methods:Forty-eight adult SD male rats were randomly divided into four groups: sham operated group(SO), superior spermatic nerve-removed group(SSN), inferior spermatic nerve-removed group(ISN) and superior and inferior nerve-removed group(SSN+ISN). Blood samples were obtained from the retro-orbital sinus before operation and on postoperative Day 1, 7, 14, 21, 28. The levels of serum testosterone(T), luteinizing hormone(LH), follicle stimulating hormone (FSH), prolactin(PRL) and estrogen(E2) were measured by RIA. Results:Serum testosterone levels dramatically decreased(P < 0.05) in all groups on postoperative Day 1, and on Day 7. The T levels of SO group returned to normal,while those of the denervated rats did not despite some increase(P < 0.05). From 14 Day on, testosterone concentrations underwent progressive decline in the denervated groups(P < 0.05), with those of SSN+ISN group significantly lower than SSN group or ISN group(P < 0.05). Serum LH concentrations were markedly elevated(P < 0.05) in all rats one day after surgery. One week later,the LH values of SO group dropped to the preoperative levels, whereas those of the denervated groups continuously increased and the LH levels of SSN+ISN group were higher than SSN group or ISN group(P < 0.05). A pronounced rise of plasma FSH were detected in all groups one day following operation(P < 0.05) and decline to the normal range occurred on Day 7. Testicular denervation also resulted in a striking rise of serum PRL(P < 0.05) and a sharp drop of E2(P < 0.05). Conclusion:The spermatic nerves may exert its modulatory effects on spermatogenesis partly via regulating sexual hormone levels.
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