文章摘要
刘秀玲,苏 阳,方 杰,肖 明,韩群颖,丁 炯.ROSA26小鼠中枢神经系统半乳糖苷酶的表达与分布[J].南京医科大学学报,2009,29(3):308~311
ROSA26小鼠中枢神经系统半乳糖苷酶的表达与分布
The expression and distribution of β-galactosidase in the central nervous system of ROSA26 mouse
投稿时间:2008-08-29  
DOI:10.7655
中文关键词: β-gal  X-Gal  转基因小鼠  中枢神经系统
英文关键词: β-gal  X-Gal  transgenic mice  central nervous system
基金项目:江苏省自然科学基金资助(BK2006576和BK2006232)
作者单位
刘秀玲 南京医科大学人体解剖学系,江苏 南京 210029 
苏 阳  
方 杰  
肖 明  
韩群颖  
丁 炯  
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中文摘要:
      目的:观察成年ROSA26小鼠中枢神经系统半乳糖苷酶(X-Gal)的表达与分布?方法:应用X-Gal组织化学结合尼氏染色及免疫组织化学双标技术研究β-gal在该嵌合体脑内的表达与分布特征?结果:X-Gal组织化学染色显示在该线系小鼠脑和脊髓中均广泛表达转基因β-gal活性产物,但以灰质为主?X-Gal 和尼氏双重染色表明,β-gal在神经元?室管膜细胞和脉络丛上皮细胞中均有表达?免疫组化进一步显示β-gal广泛表达于神经元中,但在星形胶质细胞中则未见表达?结论:ROSA26小鼠中枢神经细胞稳定而广泛的表达β-gal, 此转基因模式动物将可用于神经细胞培养?移植及其它相关神经生物学研究?
英文摘要:
      Objective:To observe the expression and distribution of β-galactosidase in the central nervous system(CNS) of adult ROSA26 mouse. Methods:X-Gal histochemistry combined with Nissl’s or immunohistochemistry double-staining was performed to localize β-gal activity in the ROSA26 mouse CNS. Results:X-Gal histochemistry staining showed that transgenic β-gal reaction products were throughout the central nervous system, but mainly located at the grey matter. X-Gal and Nissl’s double-staining showed that β-gal expressed in neurons, ependymal cells and choroid plexus cells. X-Gal histochemistry combined with immunohistochemistry further revealed that β-galactosidase was ubiquitous expression in neurons except astrocytes. Conclusion:β-gal is extensively and stably expressed in the ROSA26 mouse CNS. This transgenic line may be useful in nerve cell culture, transplantation and other neurobiological studies.
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