文章摘要
孙 勇,顾国文,李相成.肠缺血预处理对大鼠肝脏缺血再灌注损伤的保护作用[J].南京医科大学学报,2009,29(3):323~327
肠缺血预处理对大鼠肝脏缺血再灌注损伤的保护作用
Intestinal ischemic preconditioning reduces hepatic ischemia-reperfusion injury in rats
投稿时间:2008-08-29  
DOI:10.7655
中文关键词: 缺血再灌注损伤  远端预处理  肝脏  小肠
英文关键词: ischemia-reperfusion injury  remote ischemia preconditioning  liver  intestine
基金项目:江苏省"科教兴卫工程"医学重点人才基金(RC2007056)
作者单位
孙 勇 南京医科大学第一附属医院肝脏外科,江苏 南京 210029 
顾国文  
李相成  
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中文摘要:
      目的:研究大鼠的肠缺血预处理对肝脏缺血再灌注损伤(HIRI)的远端保护作用及其机制?方法:30只大鼠随机分为3组,假手术组(Sham组)仅做肝门分离;缺血再灌注组(IR组)采用肝门阻断法(Pringle’s法)缺血30 min,再灌注120 min,建立缺血再灌注模型;远端预处理组(RIPC组)阻断肠系膜上动脉10 min,再灌注10 min,然后同IR组?分别取门静脉血清测肝酶(ALT?AST)?内毒素;取肝?肠组织分别作病理学检查?MPO活性测定;取肝组织作免疫组化TNF-α和细胞间黏附分子-1(ICAM-1)测定?结果:RIPC组较IR组:①血清ALT?AST?内毒素减少(P < 0.05);②肝和肠组织损伤病理评分?MPO活性降低(P < 0.05);③肝组织内TNF-α和ICAM-1表达减少?结论:对大鼠肠的缺血预处理可以发挥远端预处理作用,减轻肝脏的缺血再灌注损伤,可能是通过减少肝脏前炎性因子的表达,减少中性粒细胞的黏附聚集而发挥作用?
英文摘要:
      Objective:To investigate the effect and mechanism of the intestinal ischemic preconditioning on hepatic ischemia-reperfusion injury in rats. Methods:Thirty SD rats were divided into three groups randomly:sham-operated group(Sham group), ischemia-reperfusion group(IR group:produced by total inflow occlusion for 30 min),and remote ischemic preconditioning group(RIPC group:induced with superior mesenteric artery occlusion before HIRI by 10 min ischemia and 10 min reperfusion). Markers of liver and intesine injury and TNF-α,ICAM-1 in liver were measured after reperfusion for 120 min. Results:IR group was associated with increased serum aminotransferases(ALT,AST), MPO activity endotoxin and the pathological injury scores. Meanwhile,in liver tissue TNF-α and ICAM-1 expression were high when IR only. There was significant improvement in these variables in RIPC group. Conclusion:RIPC of the intestine reduced HIRI which may depend on the decrease of pro-inflammatory and activity of neutrophils.
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