文章摘要
赵 强,张馥敏,王连生,王昭军,马文珠.Toll样受体4及其配体脂多糖对骨髓间充质干细胞增殖的影响[J].南京医科大学学报,2009,29(3):345~349
Toll样受体4及其配体脂多糖对骨髓间充质干细胞增殖的影响
Effects on proliferation of mesenchymal stem cells induced by Toll like receptor4 and its ligand lipopolysaccharides
投稿时间:2008-07-29  
DOI:10.7655
中文关键词: 骨髓间充质干细胞  脂多糖  Toll样受体4  增殖
英文关键词: lipopolysaccharides  mesenchymal stem cells  Toll-like receptor4  proliferation
基金项目:江苏省科委自然科学基金(BK2007254);人事部留学人员科研择优项目(DG216D5021)
作者单位
赵 强 南京医科大学第一附属医院心内科,江苏 南京 210029 
张馥敏  
王连生  
王昭军  
马文珠  
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中文摘要:
      目的:观察不同浓度脂多糖(lipopolysaccharides,LPS)对离体小鼠骨髓间充质干细胞(mesenchymal stem cells,MSCs)增殖功能的影响?方法:分别采取野生型和Toll样受体4(toll like receptor4,TLR4)基因缺失小鼠骨髓以贴壁分离法培养骨髓间充质干细胞,取第3~6代的MSCs,通过流式细胞术检测MSCs表面标志显示CD34?CD45阴性;CD105?CD29?CD44阳性?以不同浓度LPS(0.1?1.0?5.0?10.0?20.0 ?滋g/mL)分别与野生型小鼠MSCs共培养24 h和48 h,采用Cell Count Kit-8比色法观察LPS对MSCs增殖功能的影响,此外采用TLR4基因缺失小鼠MSCs与LPS 1.0 ?滋g/ml共培养,探讨LPS促进MSCs增殖的可能机制?结果:LPS(0.1~5.0 ?滋g/ml)能显著提高离体野生型小鼠MSCs的增殖能力(P < 0.05),LPS浓度在5.0 ?滋g/ml时对MSCs增殖功能影响最大,随着LPS浓度的继续增大,MSCs的增殖功能反呈下降趋势,但LPS 20.0 ?滋g/ml组增殖能力仍高于对照组(P > 0.05);LPS对LR 4基因缺失小鼠MSCs增殖能力无显著影响?结论:适量的LPS能增加MSCs的增殖能力,机制可能是通过激活Toll样受体信号通路实现的?
英文摘要:
      Objective:To observe the effects of lipopolysaccharides(LPS) of different concentrations on the proliferation of mesenchymal stem cells(MSCs). Methods:Mesenchymal stem cells(MSCs) were isolated from wild and TLR4 gene deletion mice respectively by method of differential adherence to plastic, and their phenotypical properties were analyzed in passage 3-6 by flowcytometer. Flowcytometry showed that MSCs were CD34-,CD45- and CD105+?CD29+?CD44+. MSCs isolated from wild mice were treated with LPS of different concentrations(0.1,1.0,5.0,10.0,20.0 ?滋g/ml). MSCs proliferation was detected by CCK-8(Cell Count Kit-8) co-cultured method at 24 h and 48 h respectively. In addition, MSCs isolated from TLR4 gene deletion mice incorpted with LPS 1.0 ?滋g/ml were used to explore the possible mechanism of promoting MSCs proliferation. Results:Low dose LPS(0.1~5.0 ?滋g/ml) strikingly improved the proliferation of MSCs in vitro(P < 0.05). The stimulation with LPS 5.0 ?滋g/ml reached maximum effects on MSCs proliferation. While the concentration of LPS was further increased more than 5.0 ?滋g/ml, the improvement effects on MSCs proliferation showed a tendency to decline, but the effect at 20.0 ?滋g/ml was still greater than those in control group(P > 0.05). There was no significant effects on the proliferation of MSCs isolated from TLR4 gene deletion mice induced by LPS. Conclusion:The present study revealed that LPS could improve the proliferation of MSCs through TLR4, and the mechanism might activate the Toll -like receptor4 pathway
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