文章摘要
吴 越,周晓玉,李婵娟.孕鼠维生素D缺乏对子鼠长骨维生素D受体蛋白表达的影响[J].南京医科大学学报,2009,29(3):361~363385
孕鼠维生素D缺乏对子鼠长骨维生素D受体蛋白表达的影响
Effects of vitamin D deficiency during pregnancy on femoral VDR protein expression of offspring
投稿时间:2008-08-19  
DOI:10.7655
中文关键词: 维生素D缺乏  股骨  子鼠  维生素D受体
英文关键词: Vitamin D deficiency  femur  offspring  VDR
基金项目:南京市医学科技发展一般性项目(ZKM06045);南京医科大学科技发展基金重点项目(06NMUZ024)
作者单位
吴 越 南京医科大学附属南京儿童医院新生儿科,江苏 南京 210008 
周晓玉 南京医科大学附属南京儿童医院新生儿科,江苏 南京 210008 
李婵娟 南京医科大学附属南京妇幼保健院妇产科,江苏 南京 210004 
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中文摘要:
      目的:研究大鼠孕期维生素D(VitD)缺乏对其子鼠长骨中维生素D受体(VDR)蛋白表达的影响?方法:成熟雌性SD大鼠36只随机分为模型组和对照组各18只,模型组以不含VitD的大鼠饲料避光喂养,对照组以标准饲料正常光照喂养?2周后与成熟SD雄性大鼠交配,分别取孕20天(E20d)?生后1天(B1d)和生后7天(B7d) 子鼠左侧股骨?用Western blotting方法检测子鼠股骨VDR蛋白水平的表达?结果:VDR蛋白在E20d?B1d和B7d子鼠股骨的表达量,模型组均显著低于对照组(P均 < 0.05)?结论:孕鼠VitD缺乏可下调子鼠长骨VDR蛋白表达水平,可能为子鼠骨骼发育不良机制之一?
英文摘要:
      Objective:To study the expression of VDR protein in the femurs of offsprings whose mothers were deficient of vitamin D. Methods:Thirty-six adult female SD rats were chosen and divided randomly into the control group(18 rats) and model group(18 rats).The control group was received vitamin D fodder with normal solarization and the model group received non-vitamin D fodder without solarization for 2 weeks. Then the female rats in two groups copulated with the male normal rats. The left femurs were collected from the 20-day embryos and the 1-day and 7-day newborns. VDR protein expression was measured by Western blotting. Results:The VDR protein expression of the model group was lower than the control group(P < 0.05). Conclusion:The change of expression of VDR protein was due to vitamin D deficiency during the pregnancy, which may be the reason of offsprings’ skeletal deformity.
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