文章摘要
杨长永,路雪芹,冯永东,龚建平.TNF-α诱导Molt-4细胞周期特异性凋亡及cyclin B1的表达[J].南京医科大学学报,2009,29(3):364~367388
TNF-α诱导Molt-4细胞周期特异性凋亡及cyclin B1的表达
TNF-α induces the specificity apoptosis of Molt-4 cells and the expression of cyclin B1
投稿时间:2008-08-13  
DOI:10.7655
中文关键词: 细胞周期特异性  细胞凋亡  细胞周期蛋白B1
英文关键词: cell cycle specificity  apoptosis  cyclin B1
基金项目:卫生部临床重点学科资助(20012537);河南大学自然科学基金资助(07YBZR030)
作者单位
杨长永 河南大学公共卫生研究所,河南 开封 475004 
路雪芹 河南大学公共卫生研究所,河南 开封 475004 
冯永东 华中科技大学同济医学院附属同济医院肿瘤研究所,湖北 武汉 430030 
龚建平 华中科技大学同济医学院附属同济医院肿瘤研究所,湖北 武汉 430030 
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中文摘要:
      目的:观察TNF-α诱导Molt-4细胞的凋亡现象以及cyclin B1的表达,探讨膜受体途径细胞凋亡机制?方法:用TNF-α处理指数生长期的Molt-4细胞;应用API?免疫印迹电泳等方法检测细胞凋亡和cyclin B1的表达?结果:Molt-4细胞经TNF-α诱导后出现了明显的细胞凋亡,细胞凋亡主要发生在细胞周期的G1期,cyclin B1在G1期呈阳性表达?结论:TNF-α诱导的细胞凋亡具有细胞周期特异性,cyclin B1在G1期细胞中表达升高可能与G1期细胞凋亡有关?
英文摘要:
      Objective:To observe phenomenon of Molt-4 cell apoptosis induced by TNF-αand expression of cyclin B1, and investigate the receptor-mediated apoptosis mechanism. Methods:Exponentially growing human leukemia cell lines(Molt-4) were treated with TNF-α. Method of API was used to detect the apoptosis, and method of western blot was applied to illustrate expression of cyclin B1. Results:Molt-4 cells which were treated by TNF-α went to apoptosis. Apoptosis was in G1-phase, and the expression of cyclin B1 was observed in G1-phase. Conclusion:Cell apoptosis induced by TNF-α was closely related to cell cycle and presented cell-cycle-specificity. The expression of cyclin B1 increased in G1-phase, which may be related to cell apoptosis in G1.
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